TY - JOUR

T1 - Transcriptomic Analysis of Radish (Raphanus sativus L.) Roots with CLE41 Overexpression

AU - Кузнецова, Ксения Андреевна

AU - Додуева, Ирина Евгеньевна

AU - Ганчева, Мария Семеновна

AU - Лутова, Людмила Алексеевна

N1 - Publisher Copyright: © 2022 by the authors.

PY - 2022/8/20

Y1 - 2022/8/20

N2 - The CLE41 peptide, like all other TRACHEARY ELEMENT DIFFERENTIATION INHIBITORY FACTOR (TDIF) family CLE peptides, promotes cell division in (pro-)cambium vascular meristem and prevents xylem differentiation. In this work, we analyzed the differential gene expression in the radish primary-growing P35S:RsCLE41-1 roots using the RNA-seq. Our analysis of transcriptomic data revealed a total of 62 differentially expressed genes between transgenic radish roots overexpressing the RsCLE41-1 gene and the glucuronidase ( GUS) gene. For genes associated with late embryogenesis, response to abscisic acid and auxin-dependent xylem cell fate determination, an increase in the expression in P35S:RsCLE41-1 roots was found. Among those downregulated, stress-associated genes prevailed. Moreover, several genes involved in xylem specification were also downregulated in the roots with RsCLE41-1 overexpression. Unexpectedly, none of the well-known targets of TDIFs, such as WOX4 and WOX14, were identified as DEGs in our experiment. Herein, we discuss a suggestion that the activation of pathways associated with desiccation resistance, which are more characteristic of late embryogenesis, in roots with RsCLE41-overexpression may be a consequence of water deficiency onset due to impaired vascular specification.

AB - The CLE41 peptide, like all other TRACHEARY ELEMENT DIFFERENTIATION INHIBITORY FACTOR (TDIF) family CLE peptides, promotes cell division in (pro-)cambium vascular meristem and prevents xylem differentiation. In this work, we analyzed the differential gene expression in the radish primary-growing P35S:RsCLE41-1 roots using the RNA-seq. Our analysis of transcriptomic data revealed a total of 62 differentially expressed genes between transgenic radish roots overexpressing the RsCLE41-1 gene and the glucuronidase ( GUS) gene. For genes associated with late embryogenesis, response to abscisic acid and auxin-dependent xylem cell fate determination, an increase in the expression in P35S:RsCLE41-1 roots was found. Among those downregulated, stress-associated genes prevailed. Moreover, several genes involved in xylem specification were also downregulated in the roots with RsCLE41-1 overexpression. Unexpectedly, none of the well-known targets of TDIFs, such as WOX4 and WOX14, were identified as DEGs in our experiment. Herein, we discuss a suggestion that the activation of pathways associated with desiccation resistance, which are more characteristic of late embryogenesis, in roots with RsCLE41-overexpression may be a consequence of water deficiency onset due to impaired vascular specification.

KW - CLE41

KW - RNA-seq

KW - Raphanus sativus

KW - desiccation tolerance

KW - differential gene expression

KW - late embryogenesis

KW - xylem specification

UR - https://www.mendeley.com/catalogue/38e73135-de66-320d-9745-da91ccf157f4/

UR - http://www.scopus.com/inward/record.url?scp=85137588961&partnerID=8YFLogxK

U2 - 10.3390/plants11162163

DO - 10.3390/plants11162163

M3 - Article

C2 - 36015466

VL - 11

JO - Plants

JF - Plants

SN - 2223-7747

IS - 16

M1 - 2163

ER -