TY - JOUR

T1 - The WOX Genes from the Intermediate Clade: Influence on theSomatic Embryogenesis in Medicago truncatula

AU - Яковлева, Дарья Васильевна

AU - Ефремова, Елена Павловна

AU - Смирнов, Кирилл Вадимович

AU - Симонова, Вероника

AU - Константинов, Захар

AU - Творогова, Варвара Евгеньевна

AU - Лутова, Людмила Алексеевна

PY - 2024/1/13

Y1 - 2024/1/13

N2 - Transcription factors from the WOX family are well-known regulators of cell proliferation and differentiation in plants. Herein, we focused on several WOX genes from the intermediate clade and checked their impact on somatic embryogenesis using the model legume object Medicago truncatula. As a result, we show that MtWOX9-1 overexpression not only stimulates somatic embryogenesis in the embryogenic M. truncatula line, as it was shown previously, but can also induce somatic embryogenesis in the non-embryogenic line. Other intermediate clade WOX, including the close paralog of MtWOX9-1, as well as WOX11 homologs, did not have any significant impact on somatic embryogenesis in our in vitro cultivation system. Together, our results give new information about the diversity of the WOX family proteins and their specific functions. These data can be used for the search of new regeneration stimulators.

AB - Transcription factors from the WOX family are well-known regulators of cell proliferation and differentiation in plants. Herein, we focused on several WOX genes from the intermediate clade and checked their impact on somatic embryogenesis using the model legume object Medicago truncatula. As a result, we show that MtWOX9-1 overexpression not only stimulates somatic embryogenesis in the embryogenic M. truncatula line, as it was shown previously, but can also induce somatic embryogenesis in the non-embryogenic line. Other intermediate clade WOX, including the close paralog of MtWOX9-1, as well as WOX11 homologs, did not have any significant impact on somatic embryogenesis in our in vitro cultivation system. Together, our results give new information about the diversity of the WOX family proteins and their specific functions. These data can be used for the search of new regeneration stimulators.

KW - Medicago truncatula

KW - WOX genes

KW - legumes

KW - morphogenic regulators

KW - somatic embryogenesis

UR - https://www.mendeley.com/catalogue/fd7073b2-1c46-3735-846b-306724df9010/

U2 - 10.3390/plants13020223

DO - 10.3390/plants13020223

M3 - Article

C2 - 38256776

VL - 13

JO - Plants

JF - Plants

SN - 2223-7747

IS - 2

M1 - 223

ER -