The article is focused on the assessment of changes in the average annual water levels of large lakes of the planet in the changing climate conditions characteristic of the recent decades. Eight large lakes, i.e. Baikal, Balkhash, Superior, Issyk-Kul, Ladoga, Onega, Ontario, and Erie, located on the territory of Eurasia and North America, were chosen as the research objects. They were selected because of the availability of a long-term observations series of the water level. As is known, long-term changes in the lakes water level result from variations in the volume of water in the reservoir. The latter depends on the ratios between the water balance components of the lake that have developed during a given year, which, in turn, reflect the climatic conditions of the respective years. The features of the water balance structure of the above-mentioned lakes and the intra-annual course of the water level are considered. The available long-term records of observational data on all selected lakes and their stations were divided into two periods: from 1960 to 1979 (the period of stationary climatic situation) and from 1980 to 2008 (the period of non-stationary climatic situation). The homogeneity and significance of trends in the long-term water level series of records have been estimated. It has been established that over the second period the nature and magnitude of the lakes water levels variations differ significantly. For lakes Balkhash, Issyk-Kul, Ladoga, Superior, and Erie, there is a general tendency for a decrease in water levels. For the remaining three lakes (Baikal, Onega, and Ontario), the opposite tendency has been noted: the levels of these lakes increased. Quantitatively, the range of changes in water levels on the lakes in question over the period of 1980 – 2008 ranged from -4 cm to +26 cm.