TY - JOUR

T1 - The role of phytohormones in response to transformation of pea lines, differing in symbiotic characteristics, and their hybrids

AU - Lutova, Ludmila A.

AU - Sharova, Natalya V.

AU - Kislin, Yevgeniy N.

PY - 1996

Y1 - 1996

N2 - This study investigated various morphogenetic processes following wounding, exogenous phytohormone application and oncotransformation in pea lines differing in symbiotic characteristics. The pea lines varied in callus and root formation abilities on medium without phytohormones. Exogenous phytohormones (auxins and cytokinins) decreased the diversity in callus formation but revealed genotypic diversity in shoot formation. Callus, and especially shoot formation capacities decreased greatly after subculture; the effect of phenotype became more visible. Transformation with Agrobacterium wild-type strains appeared to be more effective inducing condition revealing contrasting differences between pea genotypes in tumor- and root formation. Pea lines with atypical morphogenetic responses to transformation were revealed. The existing differences between lines for root and tumor formation allowed genetic analysis of these traits. F 1 hybrids were uniform with segregation in the F 2. The capacity for root formation was inherited as a dominant trait, but tumor formation was a recessive trait. Using exogenous phytohormones, phenocopies of the typical morphogenetic responses to transformation were obtained. The results suggest an interdependence of these morphogenetic processes with the endogenous phytohormone balance as determined by plant genotype.

AB - This study investigated various morphogenetic processes following wounding, exogenous phytohormone application and oncotransformation in pea lines differing in symbiotic characteristics. The pea lines varied in callus and root formation abilities on medium without phytohormones. Exogenous phytohormones (auxins and cytokinins) decreased the diversity in callus formation but revealed genotypic diversity in shoot formation. Callus, and especially shoot formation capacities decreased greatly after subculture; the effect of phenotype became more visible. Transformation with Agrobacterium wild-type strains appeared to be more effective inducing condition revealing contrasting differences between pea genotypes in tumor- and root formation. Pea lines with atypical morphogenetic responses to transformation were revealed. The existing differences between lines for root and tumor formation allowed genetic analysis of these traits. F 1 hybrids were uniform with segregation in the F 2. The capacity for root formation was inherited as a dominant trait, but tumor formation was a recessive trait. Using exogenous phytohormones, phenocopies of the typical morphogenetic responses to transformation were obtained. The results suggest an interdependence of these morphogenetic processes with the endogenous phytohormone balance as determined by plant genotype.

KW - Agrobacterium transformation

KW - Pea

KW - phytohormones

KW - tumor and root formation

UR - http://www.scopus.com/inward/record.url?scp=0029762661&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0029762661

VL - 21

SP - 61

EP - 80

JO - Symbiosis

JF - Symbiosis

SN - 0334-5114

IS - 1

ER -