TY - JOUR

T1 - The role of Medicago lupulina interaction with Rhizophagus irregularis in the determination of root metabolome at early stages of AM symbiosis

AU - Yurkov, Andrey P.

AU - Puzanskiy, Roman K.

AU - Kryukov, Alexey A.

AU - Gorbunova, Anastasiia O.

AU - Kudriashova, Tatyana R.

AU - Jacobi, Lidija M.

AU - Kozhemyakov, Andrei P.

AU - Romanyuk, Daria A.

AU - Aronova, Ekaterina B.

AU - Avdeeva, Galina S.

AU - Yemelyanov, Vladislav V.

AU - Shavarda, Alexey L.

AU - Shishova, Maria F.

N1 - Publisher Copyright: © 2022 by the authors.

PY - 2022/9/7

Y1 - 2022/9/7

N2 - The nature of plant-fungi interaction at early stages of arbuscular mycorrhiza (AM) development is still a puzzling problem. To investigate the processes behind this interaction, we used the Medicago lupulina MlS-1 line that forms high-efficient AM symbiosis with Rhizophagus irregularis. AM fungus actively colonizes the root system of the host plant and contributes to the formation of effective AM as characterized by a high mycorrhizal growth response (MGR) in the host plant. The present study is aimed at distinguishing the alterations in the M. lupulina root metabolic profile as an indicative marker of effective symbiosis. We examined the root metabolome at the 14th and 24th day after sowing and inoculation (DAS) with low substrate phosphorus levels. A GS-MS analysis detected 316 metabolites. Results indicated that profiles of M. lupulina root metabolites differed from those in leaves previously detected. The roots contained fewer sugars and organic acids. Hence, compounds supporting the growth of mycorrhizal fungus (especially amino acids, specific lipids, and carbohydrates) accumulated, and their presence coincided with intensive development of AM structures. Mycorrhization determined the root metabolite profile to a greater extent than host plant development. The obtained data highlight the importance of active plant-fungi metabolic interaction at early stages of host plant development for the determination of symbiotic efficiency.

AB - The nature of plant-fungi interaction at early stages of arbuscular mycorrhiza (AM) development is still a puzzling problem. To investigate the processes behind this interaction, we used the Medicago lupulina MlS-1 line that forms high-efficient AM symbiosis with Rhizophagus irregularis. AM fungus actively colonizes the root system of the host plant and contributes to the formation of effective AM as characterized by a high mycorrhizal growth response (MGR) in the host plant. The present study is aimed at distinguishing the alterations in the M. lupulina root metabolic profile as an indicative marker of effective symbiosis. We examined the root metabolome at the 14th and 24th day after sowing and inoculation (DAS) with low substrate phosphorus levels. A GS-MS analysis detected 316 metabolites. Results indicated that profiles of M. lupulina root metabolites differed from those in leaves previously detected. The roots contained fewer sugars and organic acids. Hence, compounds supporting the growth of mycorrhizal fungus (especially amino acids, specific lipids, and carbohydrates) accumulated, and their presence coincided with intensive development of AM structures. Mycorrhization determined the root metabolite profile to a greater extent than host plant development. The obtained data highlight the importance of active plant-fungi metabolic interaction at early stages of host plant development for the determination of symbiotic efficiency.

KW - Medicago lupulina; arbuscular mycorrhiza; Rhizophagus irregularis; symbiotic efficiency; plant development; physiological stage; root; metabolic profile

KW - Medicago lupulina

KW - Rhizophagus irregularis

KW - arbuscular mycorrhiza

KW - metabolic profile

KW - physiological stage

KW - plant development

KW - root

KW - symbiotic efficiency

UR - http://www.scopus.com/inward/record.url?scp=85138652661&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/1216528b-9d55-3cfb-b788-02a15f3e696a/

U2 - https://doi.org/10.3390/plants11182338

DO - https://doi.org/10.3390/plants11182338

M3 - Article

C2 - 36145739

VL - 11

JO - Plants

JF - Plants

SN - 2223-7747

IS - 18

M1 - 2338

ER -