Результаты исследований: Научные публикации в периодических изданиях › Краткий обзор › Рецензирование
The Rhodobacter capsulatus genome. / Haselkorn, Robert; Lapidus, Alla; Kogan, Yakov; Vlcek, Cestmir; Paces, Jan; Paces, Vaclav; Ulbrich, Pavel; Pecenkova, Tamara; Rebrekov, Denis; Milgram, Arthur; Mazur, Mikhail; Cox, Randal; Kyrpides, Nikos; Kapatral, Vinayak; Los, Tamara; Lykidis, Athanasios; Reznik, Gary; Vasieva, Olga; Fonstein, Michael.
в: Photosynthesis Research, Том 70, № 1, 2001, стр. 43-52.Результаты исследований: Научные публикации в периодических изданиях › Краткий обзор › Рецензирование
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TY - JOUR
T1 - The Rhodobacter capsulatus genome
AU - Haselkorn, Robert
AU - Lapidus, Alla
AU - Kogan, Yakov
AU - Vlcek, Cestmir
AU - Paces, Jan
AU - Paces, Vaclav
AU - Ulbrich, Pavel
AU - Pecenkova, Tamara
AU - Rebrekov, Denis
AU - Milgram, Arthur
AU - Mazur, Mikhail
AU - Cox, Randal
AU - Kyrpides, Nikos
AU - Kapatral, Vinayak
AU - Los, Tamara
AU - Lykidis, Athanasios
AU - Reznik, Gary
AU - Vasieva, Olga
AU - Fonstein, Michael
N1 - Funding Information: Early stages of this project were supported by the Department of Energy and a grant from the National Science Foundation for international cooperation with the sequencing group in Prague. Work in Prague was additionally supported by grant number MSM 223300006.
PY - 2001
Y1 - 2001
N2 - The genome of Rhodobacter capsulatus has been completely sequenced. It consists of a single chromosome containing 3.5 Mb and a circular plasmid of 134 kb. This effort, started in 1992, began with a fine-structure restriction map of an overlapping set of cosmids that covered the genome. Cosmid sequencing led to a gapped genome that was filled by primer walking on the chromosome and by using lambda clones. Methods had to be developed to handle strong stops in the high GC (68%) inserts. Annotation was done with the ERGO system at Integrated Genomics, as was the reconstruction of the cell's metabolism. It was possible to recognize 3709 orfs of which functional assignments could be made with high confidence to 2392 (65%). Unusual features include the presence of numerous cryptic phage genomes embedded in the chromosome.
AB - The genome of Rhodobacter capsulatus has been completely sequenced. It consists of a single chromosome containing 3.5 Mb and a circular plasmid of 134 kb. This effort, started in 1992, began with a fine-structure restriction map of an overlapping set of cosmids that covered the genome. Cosmid sequencing led to a gapped genome that was filled by primer walking on the chromosome and by using lambda clones. Methods had to be developed to handle strong stops in the high GC (68%) inserts. Annotation was done with the ERGO system at Integrated Genomics, as was the reconstruction of the cell's metabolism. It was possible to recognize 3709 orfs of which functional assignments could be made with high confidence to 2392 (65%). Unusual features include the presence of numerous cryptic phage genomes embedded in the chromosome.
KW - Annotation
KW - Comparative genomics
KW - DNA sequence
KW - Metabolic reconstruction
UR - http://www.scopus.com/inward/record.url?scp=18244394076&partnerID=8YFLogxK
U2 - 10.1023/A:1013883807771
DO - 10.1023/A:1013883807771
M3 - Short survey
AN - SCOPUS:18244394076
VL - 70
SP - 43
EP - 52
JO - Photosynthesis Research
JF - Photosynthesis Research
SN - 0166-8595
IS - 1
ER -
ID: 90041799