TY - JOUR

T1 - The possibility of using xenogeneic phagocytes in wound treatment

AU - Yakovlev, Andrey

AU - Tulin, Dmitry

AU - Savva, Anna

AU - Kruglikova, Anastasia

N1 - Publisher Copyright: Copyright: © 2022 Yakovlev et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PY - 2022/1/31

Y1 - 2022/1/31

N2 - Metamorphosis in the insect larva is associated with disintegration, engulf and digestion of larval tissues. These processes are accompanied by a significant shift in physiological parameters like high activity of hydrolytic enzymes and decrease of pH. In the way, the metamorphosing larva resembles the processes occurring in the wound at the stage of inflammation. Based on this thesis, we put forward the idea of the possibility of using insect phagocytes in the wound treatment. The search for a suitable insect cell line and the study of its properties were the purpose of the work. The abilities of insect phagocytes to retain viability and functional activity under conditions physiological for humans were also investigated. We found that blue blowfly Calliphora vicina larvae had histolysocytes, a specialized population of professional phagocytes involved in the histolysis. In vitro, histolysocytes possess high phagocytic activity to fragments of vertebrate soft tissues and debris. These cells retain viability and functional activity for a long time under conditions that are physiological for vertebrate cells. Moreover histolysocytes can realize the humoral control over the bacteria through the synthesis of antimicrobial peptides. So histolysocytes have the potential to be used as xenogeneic phagocytes in the wound treatment. The data obtained allow proceeding to experiments on laboratory animals for studying the effect of such therapy on the wound healing process.

AB - Metamorphosis in the insect larva is associated with disintegration, engulf and digestion of larval tissues. These processes are accompanied by a significant shift in physiological parameters like high activity of hydrolytic enzymes and decrease of pH. In the way, the metamorphosing larva resembles the processes occurring in the wound at the stage of inflammation. Based on this thesis, we put forward the idea of the possibility of using insect phagocytes in the wound treatment. The search for a suitable insect cell line and the study of its properties were the purpose of the work. The abilities of insect phagocytes to retain viability and functional activity under conditions physiological for humans were also investigated. We found that blue blowfly Calliphora vicina larvae had histolysocytes, a specialized population of professional phagocytes involved in the histolysis. In vitro, histolysocytes possess high phagocytic activity to fragments of vertebrate soft tissues and debris. These cells retain viability and functional activity for a long time under conditions that are physiological for vertebrate cells. Moreover histolysocytes can realize the humoral control over the bacteria through the synthesis of antimicrobial peptides. So histolysocytes have the potential to be used as xenogeneic phagocytes in the wound treatment. The data obtained allow proceeding to experiments on laboratory animals for studying the effect of such therapy on the wound healing process.

KW - Animals

KW - Anti-Infective Agents/pharmacology

KW - Cell Differentiation/drug effects

KW - Chickens

KW - Diptera

KW - Hemocytes/drug effects

KW - Inclusion Bodies/drug effects

KW - Models, Biological

KW - Phagocytes/physiology

KW - Phagocytosis/drug effects

KW - Pupa/drug effects

KW - Swine

KW - Wound Healing/drug effects

UR - http://www.scopus.com/inward/record.url?scp=85124056837&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/a53e93bc-b08b-3979-9830-c86289e0b749/

U2 - 10.1371/journal.pone.0263256

DO - 10.1371/journal.pone.0263256

M3 - Article

C2 - 35100296

AN - SCOPUS:85124056837

VL - 17

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 1

M1 - e0263256

ER -