The lichens of the Zapadny Kotlin Protected Area and neighbouring area of the fort «Rif» (Saint Petersburg, Russia) have been investigated for the first time. Altogether 181 species of lichen-forming, lichenicolous and non-lichenized fungi are reported from the protected area. Bacidina sulphurella (Samp.) M. Hauck et V. Wirth is new for Russia, Arthonia phaeophysciae Grube et Matzer is published for the first time for North-Western European Russia, and 10 species are new to St. Petersburg. Three species included in the Red Data Book of St. Petersburg (Prilozhenie…, 2014) are known from the Zapadny Kotlin Protected Area: Arthonia helvola (Nyl.) Nyl., Pleurosticta acetabulum (Neck.) Elix et Lumbsch and Ramalina dilacerata (Hoffm.) Hoffm. In spite of maritime position, there are no specific maritime elements in the lichen flora of this area.