The influence of mesenchymal stromal cells on B-cell line growth and immunoglobulin synthesis

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The influence of mesenchymal stromal cells on B-cell line growth and immunoglobulin synthesis. / Samoylovich, M. P.; Pinevich, A. A.; Shashkova, O. A.; Vartanian, N. L.; Kiseleva, L. N.; Klimovich, V. B.

в: Cell and Tissue Biology, Том 7, № 3, 01.05.2013, стр. 227-234.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

Author

Samoylovich, M. P. ; Pinevich, A. A. ; Shashkova, O. A. ; Vartanian, N. L. ; Kiseleva, L. N. ; Klimovich, V. B. / The influence of mesenchymal stromal cells on B-cell line growth and immunoglobulin synthesis. в: Cell and Tissue Biology. 2013 ; Том 7, № 3. стр. 227-234.

BibTeX

@article{835aaabef37a4a2eae80a43f7e4894d5,

title = "The influence of mesenchymal stromal cells on B-cell line growth and immunoglobulin synthesis",

abstract = "There are conflicting data on the influence of mesenchymal stromal cells (MSCs) on B-lymphocyte growth, differentiation, and immunoglobulin (Ig) production. The aim of this study was to investigate the influence of MSCs derived from the adipose tissue of healthy donors and cancer patients on the proliferation and Ig synthesis of the lymphoblastoid cell line Namalva and myeloma cell line U266. Cocultivation of Namalva cells with MSCs stimulated their proliferation and decreased the doubling time and minimal inoculation dose necessary for cloning of these lymphoblastoid cells. The presence of MSCs supported the survival and proliferation of Namalva cells cultivated in growth factor-deficient medium. MSCs also stimulated the proliferation of U266 myeloma cells. MSCs derived from adipose tissue of both healthy donors and patients with breast cancer effectively stimulated cell proliferation of B-cell lines. The presence of MSCs in mixed cultures had no influence on the production of IgM or IgE by Namalva or U266 cells, respectively. Cocultivation of Namalva or U266 cells with MSCs provided tight contacts between cells of both types.",

keywords = "B-cell lines, cocultivation, Ig synthesis, mesenchymal stromal cells",

author = "Samoylovich, {M. P.} and Pinevich, {A. A.} and Shashkova, {O. A.} and Vartanian, {N. L.} and Kiseleva, {L. N.} and Klimovich, {V. B.}",

year = "2013",

month = may,

day = "1",

doi = "10.1134/S1990519X13030103",

language = "English",

volume = "7",

pages = "227--234",

journal = "Cell and Tissue Biology",

issn = "1990-519X",

publisher = "МАИК {"}Наука/Интерпериодика{"}",

number = "3",

}

RIS

TY - JOUR

T1 - The influence of mesenchymal stromal cells on B-cell line growth and immunoglobulin synthesis

AU - Samoylovich, M. P.

AU - Pinevich, A. A.

AU - Shashkova, O. A.

AU - Vartanian, N. L.

AU - Kiseleva, L. N.

AU - Klimovich, V. B.

PY - 2013/5/1

Y1 - 2013/5/1

N2 - There are conflicting data on the influence of mesenchymal stromal cells (MSCs) on B-lymphocyte growth, differentiation, and immunoglobulin (Ig) production. The aim of this study was to investigate the influence of MSCs derived from the adipose tissue of healthy donors and cancer patients on the proliferation and Ig synthesis of the lymphoblastoid cell line Namalva and myeloma cell line U266. Cocultivation of Namalva cells with MSCs stimulated their proliferation and decreased the doubling time and minimal inoculation dose necessary for cloning of these lymphoblastoid cells. The presence of MSCs supported the survival and proliferation of Namalva cells cultivated in growth factor-deficient medium. MSCs also stimulated the proliferation of U266 myeloma cells. MSCs derived from adipose tissue of both healthy donors and patients with breast cancer effectively stimulated cell proliferation of B-cell lines. The presence of MSCs in mixed cultures had no influence on the production of IgM or IgE by Namalva or U266 cells, respectively. Cocultivation of Namalva or U266 cells with MSCs provided tight contacts between cells of both types.

AB - There are conflicting data on the influence of mesenchymal stromal cells (MSCs) on B-lymphocyte growth, differentiation, and immunoglobulin (Ig) production. The aim of this study was to investigate the influence of MSCs derived from the adipose tissue of healthy donors and cancer patients on the proliferation and Ig synthesis of the lymphoblastoid cell line Namalva and myeloma cell line U266. Cocultivation of Namalva cells with MSCs stimulated their proliferation and decreased the doubling time and minimal inoculation dose necessary for cloning of these lymphoblastoid cells. The presence of MSCs supported the survival and proliferation of Namalva cells cultivated in growth factor-deficient medium. MSCs also stimulated the proliferation of U266 myeloma cells. MSCs derived from adipose tissue of both healthy donors and patients with breast cancer effectively stimulated cell proliferation of B-cell lines. The presence of MSCs in mixed cultures had no influence on the production of IgM or IgE by Namalva or U266 cells, respectively. Cocultivation of Namalva or U266 cells with MSCs provided tight contacts between cells of both types.

KW - B-cell lines

KW - cocultivation

KW - Ig synthesis

KW - mesenchymal stromal cells

UR - http://www.scopus.com/inward/record.url?scp=84878804290&partnerID=8YFLogxK

U2 - 10.1134/S1990519X13030103

DO - 10.1134/S1990519X13030103

M3 - Article

C2 - 23662578

AN - SCOPUS:84878804290

VL - 7

SP - 227

EP - 234

JO - Cell and Tissue Biology

JF - Cell and Tissue Biology

SN - 1990-519X

IS - 3

ER -

ID: 36330033