The aim of the study was to investigate the influence of central fatigue and motor activity on sensory gating characteristics. The P50 component in a standard paired-click paradigm was used as a sensory gating index. The main attention was devoted to the P50 peak latency change in the fatigue development. It was found that the sustained muscle load leading to the fatigue development significantly reduced sensory gating and the P50 peak latency in response to the second stimulus of the pair. This may be due to the inhibitory process suppression underlying the sensory gating in the development of central fatigue.