TY - JOUR

T1 - The effects of Rhizophagus irregularis inoculation on transcriptome of Medicago lupulina leaves at early vegetative and flowering stages of plant development

AU - Юрков, Андрей Павлович

AU - Афонин, А.М.

AU - Крюков, Алексей

AU - Горбунова, А.О.

AU - Кудряшова, Татьяна

AU - Ковальчук, Анастасия

AU - Горенкова, Анастасия Игоревна

AU - Богданова, Екатерина Михайловна

AU - Косульников, Юрий

AU - Лактионов, Юрий

AU - Кожемяков, А.П.

AU - Романюк, Дарья Андреевна

AU - Жуков, Владимир Александрович

AU - Пузанский, Роман Константинович

AU - Емельянов, Владислав Владимирович

AU - Шишова, Мария Федоровна

PY - 2023/10/15

Y1 - 2023/10/15

N2 - The study is aimed at revealing the effects of Rhizophagus irregularis inoculation on the transcriptome of Medicago lupulina leaves at the early (second leaf formation) and later (flowering) stages of plant development. A pot experiment was conducted under conditions of low phosphorus (P) level in the substrate. M. lupulina plants were characterized by high mycorrhizal growth response and mycorrhization parameters. Library sequencing was performed on the Illumina HiseqXTen platform. Significant changes in the expression of 4863 (padj < 0.01) genes from 34049 functionally annotated genes were shown by Massive Analysis of cDNA Ends (MACE-Seq). GO enrichment analysis using the Kolmogorov–Smirnov test was performed, and 244 functional GO groups wereidentified, including genes contributing to the development of effective AM symbiosis. The Mercator online tool was used to assign functional classes of differentially expressed genes (DEGs). The early stage was characterized by the presence of six functional classes that included only upregulated GO groups, such as genes of carbohydrate metabolism, cellular respiration, nutrient uptake, photosynthesis, protein biosynthesis, and solute transport. At the later stage (flowering), the number of stimulated GO groups was reduced to photosynthesis and protein biosynthesis. All DEGs of the GO:0016036 group were downregulated because AM plants had higher resistance to phosphate starvation. For the first time, the upregulation of genes encoding thioredoxin in AM plant leaves was shown. It was supposed to reduce ROS level and thus, consequently, enhance the mechanisms of antioxidant protection in M. lupulina plants under conditions of low phosphorus level. Taken together, the obtained results indicate genes that are the most important for the effective symbiosiswith M. lupulina and might be engaged in other plant species. Plants

AB - The study is aimed at revealing the effects of Rhizophagus irregularis inoculation on the transcriptome of Medicago lupulina leaves at the early (second leaf formation) and later (flowering) stages of plant development. A pot experiment was conducted under conditions of low phosphorus (P) level in the substrate. M. lupulina plants were characterized by high mycorrhizal growth response and mycorrhization parameters. Library sequencing was performed on the Illumina HiseqXTen platform. Significant changes in the expression of 4863 (padj < 0.01) genes from 34049 functionally annotated genes were shown by Massive Analysis of cDNA Ends (MACE-Seq). GO enrichment analysis using the Kolmogorov–Smirnov test was performed, and 244 functional GO groups wereidentified, including genes contributing to the development of effective AM symbiosis. The Mercator online tool was used to assign functional classes of differentially expressed genes (DEGs). The early stage was characterized by the presence of six functional classes that included only upregulated GO groups, such as genes of carbohydrate metabolism, cellular respiration, nutrient uptake, photosynthesis, protein biosynthesis, and solute transport. At the later stage (flowering), the number of stimulated GO groups was reduced to photosynthesis and protein biosynthesis. All DEGs of the GO:0016036 group were downregulated because AM plants had higher resistance to phosphate starvation. For the first time, the upregulation of genes encoding thioredoxin in AM plant leaves was shown. It was supposed to reduce ROS level and thus, consequently, enhance the mechanisms of antioxidant protection in M. lupulina plants under conditions of low phosphorus level. Taken together, the obtained results indicate genes that are the most important for the effective symbiosiswith M. lupulina and might be engaged in other plant species. Plants

KW - Medicago lupulina

KW - arbuscular mycorrhiza

KW - Rhizophagus irregularis

KW - symbiotic efficiency

KW - mycorrhizal growth response

KW - plant development

KW - physiological stage

KW - leaf

KW - transcriptome

KW - phosphorus deficiency

KW - Medicago lupulina

KW - Rhizophagus irregularis

KW - arbuscular mycorrhiza

KW - leaf

KW - mycorrhizal growth response

KW - phosphorus deficiency

KW - physiological stage

KW - plant development

KW - symbiotic efficiency

KW - transcriptome

UR - https://www.mendeley.com/catalogue/aac53845-1621-3f97-98a2-1e07b859f887/

U2 - 10.3390/plants12203580

DO - 10.3390/plants12203580

M3 - Article

C2 - 37896043

VL - 12

JO - Plants

JF - Plants

SN - 2223-7747

IS - 20

M1 - 3580

ER -