Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
The dynamics of the formation of cytochrome C complexes with anionic lipids and the mechanism of the production of lipid radicais catalyzed by these complexes. / Vladimirov, G. K.; Nesterova, A. M.; Levkina, A. A.; Osipov, A. N.; Teselkin, Y. O.; Kovalchuk, M. V.; Vladimirov, Yu A.
в: Biologicheskie Membrany, Том 37, № 4, 2020, стр. 287-298.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - The dynamics of the formation of cytochrome C complexes with anionic lipids and the mechanism of the production of lipid radicais catalyzed by these complexes
AU - Vladimirov, G. K.
AU - Nesterova, A. M.
AU - Levkina, A. A.
AU - Osipov, A. N.
AU - Teselkin, Y. O.
AU - Kovalchuk, M. V.
AU - Vladimirov, Yu A.
N1 - Publisher Copyright: © 2020 Russian Academy of Sciences, Institute of Archaeology. All rights reserved.
PY - 2020
Y1 - 2020
N2 - In the present work, the recorded kinetics of chemiluminescence enhanced by coumarin 334 indicated that the complexes of cytochrome c with anionic lipids (Cyt-AL), such as cardiolipin (CL) and phosphatidic acid (PA), when added to mitochondrial and cytoplasmic membranes, are capable of catalysing the reactions of lipid free radical formation. The effect of Cyt-CL complex was studied on mitochondria isolated from the liv-er of male Wistar rats and male outbred mice, as well as on erythrocytes isolated from the blood of male Wistar rats. It was shown that Cyt-PA complex exerts a peroxidase effect similar to that of Cyt-CL. Upon exposure to Cyt-CL, the formation of free radicais was more prominent in damaged mitochondria (mitochondria shocked by freeze-thawing and washed by several precipitation-resuspension cycles) than in intact mitochondria. This suggests that Cyt-CL does not pass through biological membranes: even if it penetrates into them, it probably gets stuck in the phospholipid bilayer. Thus, the previously discovered cytotoxic effect of Cyt-CL on câncer cells most likely can be attributed to peroxidation of the cytoplasmic membrane but not of the inner membrane of mitochondria.
AB - In the present work, the recorded kinetics of chemiluminescence enhanced by coumarin 334 indicated that the complexes of cytochrome c with anionic lipids (Cyt-AL), such as cardiolipin (CL) and phosphatidic acid (PA), when added to mitochondrial and cytoplasmic membranes, are capable of catalysing the reactions of lipid free radical formation. The effect of Cyt-CL complex was studied on mitochondria isolated from the liv-er of male Wistar rats and male outbred mice, as well as on erythrocytes isolated from the blood of male Wistar rats. It was shown that Cyt-PA complex exerts a peroxidase effect similar to that of Cyt-CL. Upon exposure to Cyt-CL, the formation of free radicais was more prominent in damaged mitochondria (mitochondria shocked by freeze-thawing and washed by several precipitation-resuspension cycles) than in intact mitochondria. This suggests that Cyt-CL does not pass through biological membranes: even if it penetrates into them, it probably gets stuck in the phospholipid bilayer. Thus, the previously discovered cytotoxic effect of Cyt-CL on câncer cells most likely can be attributed to peroxidation of the cytoplasmic membrane but not of the inner membrane of mitochondria.
KW - Apoptosis
KW - Cardiolipin
KW - Complex of cytochrome c with anionic lipids
KW - Cytochrome c
KW - Lipid peroxidation
KW - Phosphatidic acid
UR - http://www.scopus.com/inward/record.url?scp=85097197333&partnerID=8YFLogxK
U2 - 10.31857/S0233475520030088
DO - 10.31857/S0233475520030088
M3 - Article
AN - SCOPUS:85097197333
VL - 37
SP - 287
EP - 298
JO - БИОЛОГИЧЕСКИЕ МЕМБРАНЫ: ЖУРНАЛ МЕМБРАННОЙ И КЛЕТОЧНОЙ БИОЛОГИИ
JF - БИОЛОГИЧЕСКИЕ МЕМБРАНЫ: ЖУРНАЛ МЕМБРАННОЙ И КЛЕТОЧНОЙ БИОЛОГИИ
SN - 0233-4755
IS - 4
ER -
ID: 88197136