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The dynamic properties of PDA-laccase films at the air-water interface. / Milyaeva, O. Yu.; Bykov, A. G.; Campbell, R. A.; Loglio, G.; Miller, R.; Noskov, B. A.

в: Colloids and Surfaces A: Physicochemical and Engineering Aspects, Том 599, 124930, 20.08.2020.

Результаты исследований: Научные публикации в периодических изданияхстатьяРецензирование

Harvard

Milyaeva, OY, Bykov, AG, Campbell, RA, Loglio, G, Miller, R & Noskov, BA 2020, 'The dynamic properties of PDA-laccase films at the air-water interface', Colloids and Surfaces A: Physicochemical and Engineering Aspects, Том. 599, 124930. https://doi.org/10.1016/j.colsurfa.2020.124930

APA

Milyaeva, O. Y., Bykov, A. G., Campbell, R. A., Loglio, G., Miller, R., & Noskov, B. A. (2020). The dynamic properties of PDA-laccase films at the air-water interface. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 599, [124930]. https://doi.org/10.1016/j.colsurfa.2020.124930

Vancouver

Milyaeva OY, Bykov AG, Campbell RA, Loglio G, Miller R, Noskov BA. The dynamic properties of PDA-laccase films at the air-water interface. Colloids and Surfaces A: Physicochemical and Engineering Aspects. 2020 Авг. 20;599. 124930. https://doi.org/10.1016/j.colsurfa.2020.124930

Author

Milyaeva, O. Yu. ; Bykov, A. G. ; Campbell, R. A. ; Loglio, G. ; Miller, R. ; Noskov, B. A. / The dynamic properties of PDA-laccase films at the air-water interface. в: Colloids and Surfaces A: Physicochemical and Engineering Aspects. 2020 ; Том 599.

BibTeX

@article{d97f0ce9b52a43e3be0a0c877b3508be,
title = "The dynamic properties of PDA-laccase films at the air-water interface",
abstract = "Polydopamine (PDA) films at the air - water interface can be easily obtained in slightly alkaline dopamine solutions using 02 dissolved in water as an oxidant but their insufficient stability limits their applications. In this work PDA films at the water surface were obtained by polymerization of dopamine hydrochloride (DA) by the enzyme laccase in a slightly acidic environment at a constant concentration of enzyme and DA concentrations in the range from 0.1 to 10 g/l. The dynamic surface elasticity, effective surface tension, film thickness and refractive index were measured as function of time to characterize the main steps of film formation. The obtained results show that laccase accelerates the polymerization process, decreases the required DA concentration for film formation, and insight from Brewster angle microscopy shows that laccase increases the resistance of the films to mechanical deformation. The dynamic surface elasticity of the obtained films is about seven times higher than the highest values for either pure PDA or pure laccase films. Laccase is inferred not only to provide crosslinking of the polymer but is also incorporated in the film resulting in its higher stability. The kinetic dependences of the surface properties allow the main steps of film formation to be distinguished: nucleation of polymer domains in a rapidly-formed protein film, coexistence of the protein and polymer domains, and their transformation into a highly dense composite film with encapsulated protein. Insight from atomic force microscopy images of films transferred to mica show that PDA fills in the gaps of spherical laccase aggregates when the DA concentration is increased and the film becomes less rough. The inclusion of laccase in PDA films significantly increases their stability.",
keywords = "Air-water interface, BAM, Dilational surface visco-elasticity, Ellipsometry, Laccase, Polydopamine, Polymerization, THIN-FILMS, NANOPARTICLES, DOPAMINE, POLYMERIZATION, POLYDOPAMINE FILMS",
author = "Milyaeva, {O. Yu.} and Bykov, {A. G.} and Campbell, {R. A.} and G. Loglio and R. Miller and Noskov, {B. A.}",
year = "2020",
month = aug,
day = "20",
doi = "10.1016/j.colsurfa.2020.124930",
language = "English",
volume = "599",
journal = "Colloids and Surfaces A: Physicochemical and Engineering Aspects",
issn = "0927-7757",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - The dynamic properties of PDA-laccase films at the air-water interface

AU - Milyaeva, O. Yu.

AU - Bykov, A. G.

AU - Campbell, R. A.

AU - Loglio, G.

AU - Miller, R.

AU - Noskov, B. A.

PY - 2020/8/20

Y1 - 2020/8/20

N2 - Polydopamine (PDA) films at the air - water interface can be easily obtained in slightly alkaline dopamine solutions using 02 dissolved in water as an oxidant but their insufficient stability limits their applications. In this work PDA films at the water surface were obtained by polymerization of dopamine hydrochloride (DA) by the enzyme laccase in a slightly acidic environment at a constant concentration of enzyme and DA concentrations in the range from 0.1 to 10 g/l. The dynamic surface elasticity, effective surface tension, film thickness and refractive index were measured as function of time to characterize the main steps of film formation. The obtained results show that laccase accelerates the polymerization process, decreases the required DA concentration for film formation, and insight from Brewster angle microscopy shows that laccase increases the resistance of the films to mechanical deformation. The dynamic surface elasticity of the obtained films is about seven times higher than the highest values for either pure PDA or pure laccase films. Laccase is inferred not only to provide crosslinking of the polymer but is also incorporated in the film resulting in its higher stability. The kinetic dependences of the surface properties allow the main steps of film formation to be distinguished: nucleation of polymer domains in a rapidly-formed protein film, coexistence of the protein and polymer domains, and their transformation into a highly dense composite film with encapsulated protein. Insight from atomic force microscopy images of films transferred to mica show that PDA fills in the gaps of spherical laccase aggregates when the DA concentration is increased and the film becomes less rough. The inclusion of laccase in PDA films significantly increases their stability.

AB - Polydopamine (PDA) films at the air - water interface can be easily obtained in slightly alkaline dopamine solutions using 02 dissolved in water as an oxidant but their insufficient stability limits their applications. In this work PDA films at the water surface were obtained by polymerization of dopamine hydrochloride (DA) by the enzyme laccase in a slightly acidic environment at a constant concentration of enzyme and DA concentrations in the range from 0.1 to 10 g/l. The dynamic surface elasticity, effective surface tension, film thickness and refractive index were measured as function of time to characterize the main steps of film formation. The obtained results show that laccase accelerates the polymerization process, decreases the required DA concentration for film formation, and insight from Brewster angle microscopy shows that laccase increases the resistance of the films to mechanical deformation. The dynamic surface elasticity of the obtained films is about seven times higher than the highest values for either pure PDA or pure laccase films. Laccase is inferred not only to provide crosslinking of the polymer but is also incorporated in the film resulting in its higher stability. The kinetic dependences of the surface properties allow the main steps of film formation to be distinguished: nucleation of polymer domains in a rapidly-formed protein film, coexistence of the protein and polymer domains, and their transformation into a highly dense composite film with encapsulated protein. Insight from atomic force microscopy images of films transferred to mica show that PDA fills in the gaps of spherical laccase aggregates when the DA concentration is increased and the film becomes less rough. The inclusion of laccase in PDA films significantly increases their stability.

KW - Air-water interface

KW - BAM

KW - Dilational surface visco-elasticity

KW - Ellipsometry

KW - Laccase

KW - Polydopamine

KW - Polymerization

KW - THIN-FILMS

KW - NANOPARTICLES

KW - DOPAMINE

KW - POLYMERIZATION

KW - POLYDOPAMINE FILMS

UR - http://www.scopus.com/inward/record.url?scp=85084334960&partnerID=8YFLogxK

U2 - 10.1016/j.colsurfa.2020.124930

DO - 10.1016/j.colsurfa.2020.124930

M3 - Article

AN - SCOPUS:85084334960

VL - 599

JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects

JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects

SN - 0927-7757

M1 - 124930

ER -

ID: 60314510