Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
The Anti-Epileptic Effects of Carbenoxolone In Vitro and In Vivo. / Volnova, Anna; Tsytsarev, Vassiliy; Ganina, Olga; Vélez-Crespo, Grace E.; Alves, Janaina M.; Ignashchenkova, Alla; Inyushin, Mikhail.
в: International Journal of Molecular Sciences, Том 23, № 2, 663, 08.01.2022.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - The Anti-Epileptic Effects of Carbenoxolone In Vitro and In Vivo
AU - Volnova, Anna
AU - Tsytsarev, Vassiliy
AU - Ganina, Olga
AU - Vélez-Crespo, Grace E.
AU - Alves, Janaina M.
AU - Ignashchenkova, Alla
AU - Inyushin, Mikhail
N1 - Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/1/8
Y1 - 2022/1/8
N2 - Gap junctions (GJs) are intercellular junctions that allow the direct transfer of ions and small molecules between neighboring cells, and GJs between astrocytes play an important role in the development of various pathologies of the brain, including regulation of the pathological neuronal synchronization underlying epileptic seizures. Recently, we found that a pathological change is observed in astrocytes during the ictal and interictal phases of 4-aminopyridin (4-AP)-elicited epileptic activity in vitro, which was correlated with neuronal synchronization and extracellular epileptic electrical activity. This finding raises the question: Does this signal depend on GJs between astrocytes? In this study we investigated the effect of the GJ blocker, carbenoxolone (CBX), on epileptic activity in vitro and in vivo. Based on the results obtained, we came to the conclusion that the astrocytic syncytium formed by GJ-associated astrocytes, which is responsible for the regulation of potassium, affects the formation of epileptic activity in astrocytes in vitro and epileptic seizure onset. This effect is probably an important, but not the only, mechanism by which CBX suppresses epileptic activity. It is likely that the mechanisms of selective inhibition of GJs between astrocytes will show important translational benefits in anti-epileptic therapies.
AB - Gap junctions (GJs) are intercellular junctions that allow the direct transfer of ions and small molecules between neighboring cells, and GJs between astrocytes play an important role in the development of various pathologies of the brain, including regulation of the pathological neuronal synchronization underlying epileptic seizures. Recently, we found that a pathological change is observed in astrocytes during the ictal and interictal phases of 4-aminopyridin (4-AP)-elicited epileptic activity in vitro, which was correlated with neuronal synchronization and extracellular epileptic electrical activity. This finding raises the question: Does this signal depend on GJs between astrocytes? In this study we investigated the effect of the GJ blocker, carbenoxolone (CBX), on epileptic activity in vitro and in vivo. Based on the results obtained, we came to the conclusion that the astrocytic syncytium formed by GJ-associated astrocytes, which is responsible for the regulation of potassium, affects the formation of epileptic activity in astrocytes in vitro and epileptic seizure onset. This effect is probably an important, but not the only, mechanism by which CBX suppresses epileptic activity. It is likely that the mechanisms of selective inhibition of GJs between astrocytes will show important translational benefits in anti-epileptic therapies.
KW - Astrocytes
KW - Carbenoxolone
KW - Epilepsy
KW - Gap junction
KW - Neural synchronization
KW - Seizures
UR - http://www.scopus.com/inward/record.url?scp=85122351617&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/5c3f41f5-9d26-3479-b9e1-24f1a0b5e861/
U2 - 10.3390/ijms23020663
DO - 10.3390/ijms23020663
M3 - Article
C2 - 35054848
AN - SCOPUS:85122351617
VL - 23
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1422-0067
IS - 2
M1 - 663
ER -
ID: 95339247