TY - CONF

T1 - The actin cytoskeleton is a target for NaCl and hydroxyl radicals in Arabidopsis thaliana root cells

AU - Pozhvanov, G.

AU - Medvedev, S.

AU - Vissenberg, K.

AU - Demidchik, V.

PY - 2014

Y1 - 2014

N2 - The effect of NaCl on root cells is a prime cause of plant damage by salinity. In Arabidopsis thaliana root cells, early effects of NaCl (20-400 mM) include the elevation of cytosolic free Ca2+, generation of hydroxyl radicals and K+ efflux, which together trigger adaptation programmes, metabolic adjustment or a programmed cell death (Demidchik et al., 2014, DOI: 10.1093/jxb/eru004). Using GFP-FABD2 Arabidopsis roots and confocal microscopy, we have found that the cytoskeleton is another prime target for NaCl. Here, we have demonstrated that 100 mM NaCl delays or stops root growth and induces actin polymerization in the root elongation zone within 10 min. Microfilament angle distribution deviated from an initial orientation (predominantly axial) to a broad spectrum with peaks at 15°, 45° and 90°. This effect was prevented by addition of polyamines (spermine and spermidine), blockers of Ca2+-permeable cation channels and scavengers of hydroxyl radicals. Therefore, it was probably related to Ca2+ influx and hyd

AB - The effect of NaCl on root cells is a prime cause of plant damage by salinity. In Arabidopsis thaliana root cells, early effects of NaCl (20-400 mM) include the elevation of cytosolic free Ca2+, generation of hydroxyl radicals and K+ efflux, which together trigger adaptation programmes, metabolic adjustment or a programmed cell death (Demidchik et al., 2014, DOI: 10.1093/jxb/eru004). Using GFP-FABD2 Arabidopsis roots and confocal microscopy, we have found that the cytoskeleton is another prime target for NaCl. Here, we have demonstrated that 100 mM NaCl delays or stops root growth and induces actin polymerization in the root elongation zone within 10 min. Microfilament angle distribution deviated from an initial orientation (predominantly axial) to a broad spectrum with peaks at 15°, 45° and 90°. This effect was prevented by addition of polyamines (spermine and spermidine), blockers of Ca2+-permeable cation channels and scavengers of hydroxyl radicals. Therefore, it was probably related to Ca2+ influx and hyd

M3 - тезисы

SP - 1

Y2 - 22 June 2014 through 26 June 2014

ER -