Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
The cyclin-dependent protein kinase Pho85 is involved in the regulation of phosphate metabolism in yeast Saccharomyces cerevisiae. Mutations in the PHO85 gene lead to constitutive synthesis of Pho5 acid phosphatase, a delay in cell growth on media containing nonfermentable carbon sources, and other pleiotropic effects. In this work, it was shown that the accumulation of respiratory incompetent cells occurs with high frequency in strains carrying pho85 mutations as early as during the first cell divisions, and the number of these cells at the early logarithmic growth phase of the culture promptly reaches virtually 100%. Cytological analysis revealed a high accumulation rate of [rho 0] cells in the background of gene pho85 that may be related to disturbances in the distribution of mitochondrial nucleoids rather than to changes in morphology of mitochondria and a delay in their transport into the bud. Genetic analysis revealed that secondary mutations pho4, pho81, pho84, and pho87 stabilize nucleoids and prevent the loss of mitochondrial DNA caused by pho85. These results provide an evidence for the influence of intracellular phosphate concentration on the inheritance of mitochondrial nucleoids, but do not exclude the possibility that the occurrence of mutation pho4 in the background of gene pho85 may change the expression level of other genes required for the stabilization of mitochondrial functions.
Язык оригинала | английский |
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Страницы (с-по) | 651-658 |
Число страниц | 8 |
Журнал | Russian Journal of Genetics |
Том | 45 |
Номер выпуска | 6 |
DOI | |
Состояние | Опубликовано - 1 июн 2009 |
ID: 36923223