TY - JOUR

T1 - Synthetically modified BSA-based heterometallic nanoparticles facilitating energy and/or electron transfer events

AU - Файзулин, Булат

AU - Корнев, Тимофей

AU - Ниритдинов, М.

AU - Холин, К.

AU - Низамеев, И.

AU - Герасимова, Татьяна Павловна

AU - Шмелев, А.

AU - Никифоров, В.

AU - Тарасов, М.

AU - Будникова, Ю.

AU - Падерина, Александра Владимировна

AU - Грачева, Елена Валерьевна

AU - Зайров, Р.

AU - Мустафина, Асия

PY - 2025/9

Y1 - 2025/9

N2 - The variety of binding sites in BSA opens up possibilities for its use in developing systems with different functionality. However, the facile methods to develop co-loaded BSA nanoparticles (BNP) with luminescent and catalytic functions have not yet been fully explored. The luminescent lipophilic Pt2+ complex loaded into BNP via its outer-sphere binding to the hydrophobic regions of unfolded BSA serves as a luminescent probe to visualize the binding of Cu2+ ions followed by the Cu2+ → Cu+ reduction step and subsequent removal of these ions from BNP by glutathione. The specificity of luminescence and electrochemical behavior of co-loaded BNP reveals both a significant mutual influence of Cu(I) and Pt(II) centers and the influence of the co-loading method on these effects. The modification of the method allows changing the amount of copper ions per BNP, the luminescence of the complex and the laccase-like activity of copper ions bound to BNP. The latter activity was demonstrated using the oxidative conversion of adrenaline to adrenochrome. The above trends indicate that co-loading of redox-active copper ions and luminescent Pt(II) complex into BNP provides a route for combining sensing and catalytic activities, which can be tuned by modifying the synthetic method.

AB - The variety of binding sites in BSA opens up possibilities for its use in developing systems with different functionality. However, the facile methods to develop co-loaded BSA nanoparticles (BNP) with luminescent and catalytic functions have not yet been fully explored. The luminescent lipophilic Pt2+ complex loaded into BNP via its outer-sphere binding to the hydrophobic regions of unfolded BSA serves as a luminescent probe to visualize the binding of Cu2+ ions followed by the Cu2+ → Cu+ reduction step and subsequent removal of these ions from BNP by glutathione. The specificity of luminescence and electrochemical behavior of co-loaded BNP reveals both a significant mutual influence of Cu(I) and Pt(II) centers and the influence of the co-loading method on these effects. The modification of the method allows changing the amount of copper ions per BNP, the luminescence of the complex and the laccase-like activity of copper ions bound to BNP. The latter activity was demonstrated using the oxidative conversion of adrenaline to adrenochrome. The above trends indicate that co-loading of redox-active copper ions and luminescent Pt(II) complex into BNP provides a route for combining sensing and catalytic activities, which can be tuned by modifying the synthetic method.

KW - Copper complexes

KW - Energy/electron transfer processes

KW - Laccase-like catalytic activity

KW - Luminescent platinum(II) complex

KW - Nanoparticles

KW - Quenching mechanisms

UR - https://www.mendeley.com/catalogue/c2de573a-3889-3196-80b0-8d15c17ad139/

U2 - 10.1016/j.ijbiomac.2025.146540

DO - 10.1016/j.ijbiomac.2025.146540

M3 - Article

VL - 321

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

SN - 0141-8130

IS - Part 3

M1 - 146540

ER -