TY - JOUR

T1 - Synthetic polycation

T2 - Polynucleotide interactions determined using liquid chromatography with short monolithic columns

AU - Platonova, Galina A.

AU - Nazarova, Olga V.

AU - Tennikova, Tatiana B.

PY - 2009

Y1 - 2009

N2 - LC on short monolithic columns (Convective Interaction Medium Disks) was applied to investigate several specially synthesized water soluble polycations of different charge type (primary, tertiary, quaternary amine), as well as a copolymer of neutral saccharide and cationic monomers, regarding their ability to form reversible complexes with DNA. For this purpose, two separation modes were used, namely, pseudo-affinity and cation-exchange chromatography. Synthetic polynucleotides, namely, polyriboadenylic acid (poly(rA)) and polyribocytidylic acid (poly(rC)), were used as approximate structural analogues of DNA. In first case, the hypothetical specific binding between dissolved polymers and polynucleotide (poly(rA) or poly(rC)), covalently attached to epoxy-bearing monolithic sorbent, has been studied and compared to the results obtained using cation exchange chromatography. Quantitative parameters of interactions between macromolecules were established using frontal elution method.

AB - LC on short monolithic columns (Convective Interaction Medium Disks) was applied to investigate several specially synthesized water soluble polycations of different charge type (primary, tertiary, quaternary amine), as well as a copolymer of neutral saccharide and cationic monomers, regarding their ability to form reversible complexes with DNA. For this purpose, two separation modes were used, namely, pseudo-affinity and cation-exchange chromatography. Synthetic polynucleotides, namely, polyriboadenylic acid (poly(rA)) and polyribocytidylic acid (poly(rC)), were used as approximate structural analogues of DNA. In first case, the hypothetical specific binding between dissolved polymers and polynucleotide (poly(rA) or poly(rC)), covalently attached to epoxy-bearing monolithic sorbent, has been studied and compared to the results obtained using cation exchange chromatography. Quantitative parameters of interactions between macromolecules were established using frontal elution method.

KW - Cation-exchange chromatography

KW - Monoliths

KW - Polyribonucleotides

KW - Pseudo-affinity chromatography

KW - Water soluble polycations

UR - http://www.scopus.com/inward/record.url?scp=70350499909&partnerID=8YFLogxK

U2 - 10.1002/jssc.200900144

DO - 10.1002/jssc.200900144

M3 - Article

C2 - 19603391

AN - SCOPUS:70350499909

VL - 32

SP - 2674

EP - 2681

JO - Journal of Separation Science

JF - Journal of Separation Science

SN - 1615-9306

IS - 15-16

ER -