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Synthesis of GnRH analogues and their application in targeted gene delivery systems. / Yablokova, T. V.; Chelushkin, P. S.; Dorosh, M. Yu; Efremov, A. M.; Orlov, S. V.; Burov, S. V.

в: Russian Journal of Bioorganic Chemistry, Том 38, № 1, 01.2012, стр. 22-29.

Результаты исследований: Научные публикации в периодических изданияхстатьяРецензирование

Harvard

Yablokova, TV, Chelushkin, PS, Dorosh, MY, Efremov, AM, Orlov, SV & Burov, SV 2012, 'Synthesis of GnRH analogues and their application in targeted gene delivery systems', Russian Journal of Bioorganic Chemistry, Том. 38, № 1, стр. 22-29. https://doi.org/10.1134/S1068162012010190

APA

Yablokova, T. V., Chelushkin, P. S., Dorosh, M. Y., Efremov, A. M., Orlov, S. V., & Burov, S. V. (2012). Synthesis of GnRH analogues and their application in targeted gene delivery systems. Russian Journal of Bioorganic Chemistry, 38(1), 22-29. https://doi.org/10.1134/S1068162012010190

Vancouver

Yablokova TV, Chelushkin PS, Dorosh MY, Efremov AM, Orlov SV, Burov SV. Synthesis of GnRH analogues and their application in targeted gene delivery systems. Russian Journal of Bioorganic Chemistry. 2012 Янв.;38(1):22-29. https://doi.org/10.1134/S1068162012010190

Author

Yablokova, T. V. ; Chelushkin, P. S. ; Dorosh, M. Yu ; Efremov, A. M. ; Orlov, S. V. ; Burov, S. V. / Synthesis of GnRH analogues and their application in targeted gene delivery systems. в: Russian Journal of Bioorganic Chemistry. 2012 ; Том 38, № 1. стр. 22-29.

BibTeX

@article{97f9f0743af9479f9509b1eb49cdec82,
title = "Synthesis of GnRH analogues and their application in targeted gene delivery systems",
abstract = "A set of GnRH analogues containing the nuclear localization signal (NLS) of the SV-40 virus was synthesized using solid phase peptide synthesis and chemical ligation techniques. Selective chemical ligation was achieved through hydrazone formation upon the interaction of NLS hydrazide and GnRH analogue modified with pyruvic acid. The efficiency of the synthesized compounds was demonstrated in experiments on transfection of various human cancer cell lines with reporter luciferase and β-galactosidase genes, as well as suicide thymidine kinase gene of HSV-1. Selectivity of the peptide-DNA complex effect on cancer cells is achieved as a result of its penetration through the cell membrane via GnRH receptor-mediated endocytosis pathway.",
keywords = "Antitumor activity, GnRH, Nuclear localization signal, Suicide gene therapy, Targeted gene delivery",
author = "Yablokova, {T. V.} and Chelushkin, {P. S.} and Dorosh, {M. Yu} and Efremov, {A. M.} and Orlov, {S. V.} and Burov, {S. V.}",
note = "Funding Information: ACKNOWLEDGMENTS The work was supported by the grant of the Minis try of Education and Science of the Russian Federa tion (State Contract no. 16.512.11.2052).",
year = "2012",
month = jan,
doi = "10.1134/S1068162012010190",
language = "English",
volume = "38",
pages = "22--29",
journal = "Russian Journal of Bioorganic Chemistry",
issn = "1068-1620",
publisher = "МАИК {"}Наука/Интерпериодика{"}",
number = "1",

}

RIS

TY - JOUR

T1 - Synthesis of GnRH analogues and their application in targeted gene delivery systems

AU - Yablokova, T. V.

AU - Chelushkin, P. S.

AU - Dorosh, M. Yu

AU - Efremov, A. M.

AU - Orlov, S. V.

AU - Burov, S. V.

N1 - Funding Information: ACKNOWLEDGMENTS The work was supported by the grant of the Minis try of Education and Science of the Russian Federa tion (State Contract no. 16.512.11.2052).

PY - 2012/1

Y1 - 2012/1

N2 - A set of GnRH analogues containing the nuclear localization signal (NLS) of the SV-40 virus was synthesized using solid phase peptide synthesis and chemical ligation techniques. Selective chemical ligation was achieved through hydrazone formation upon the interaction of NLS hydrazide and GnRH analogue modified with pyruvic acid. The efficiency of the synthesized compounds was demonstrated in experiments on transfection of various human cancer cell lines with reporter luciferase and β-galactosidase genes, as well as suicide thymidine kinase gene of HSV-1. Selectivity of the peptide-DNA complex effect on cancer cells is achieved as a result of its penetration through the cell membrane via GnRH receptor-mediated endocytosis pathway.

AB - A set of GnRH analogues containing the nuclear localization signal (NLS) of the SV-40 virus was synthesized using solid phase peptide synthesis and chemical ligation techniques. Selective chemical ligation was achieved through hydrazone formation upon the interaction of NLS hydrazide and GnRH analogue modified with pyruvic acid. The efficiency of the synthesized compounds was demonstrated in experiments on transfection of various human cancer cell lines with reporter luciferase and β-galactosidase genes, as well as suicide thymidine kinase gene of HSV-1. Selectivity of the peptide-DNA complex effect on cancer cells is achieved as a result of its penetration through the cell membrane via GnRH receptor-mediated endocytosis pathway.

KW - Antitumor activity

KW - GnRH

KW - Nuclear localization signal

KW - Suicide gene therapy

KW - Targeted gene delivery

UR - http://www.scopus.com/inward/record.url?scp=84861151348&partnerID=8YFLogxK

U2 - 10.1134/S1068162012010190

DO - 10.1134/S1068162012010190

M3 - Article

AN - SCOPUS:84861151348

VL - 38

SP - 22

EP - 29

JO - Russian Journal of Bioorganic Chemistry

JF - Russian Journal of Bioorganic Chemistry

SN - 1068-1620

IS - 1

ER -

ID: 86146771