TY - JOUR

T1 - Synthesis, biocompatibility and biological activity of a graphene oxide-folic acid conjugate for cytarabine delivery

AU - Ageev, S.V.

AU - Semenov, K.N.

AU - Shemchuk, O.S.

AU - Iurev, G.O.

AU - Andoskin, P.A.

AU - Rumiantsev, A.M.

AU - Sambuk, E.V.

AU - Kozhukhov, P.K.

AU - Maistrenko, D.N.

AU - Molchanov, O.E.

AU - Murin, I.V.

AU - Mazur, A.S.

AU - Sharoyko, V.V.

N1 - Цитирования:1 Export Date: 19 October 2024 CODEN: CPEAE Адрес для корреспонденции: Semenov, K.N.6–8 L'va Tolstogo Street, Russian Federation; эл. почта: knsemenov@gmail.com Химические вещества/CAS: collagen, 9007-34-5; cytarabine, 147-94-4, 69-74-9; folic acid, 59-30-3, 6484-89-5 Сведения о финансировании: Saint Petersburg State University, SPbU Сведения о финансировании: 123021300231-8 Текст о финансировании 1: The work was carried out with the financial support of the Ministry of Health of the Russian Federation \u2018Molecular design and creation of drugs based on conjugates of carbon nanostructures, vectors of targeted delivery and cytotoxic agents for inactivation of stem tumour cells and components of the tumour microenvironment\u2019, No. EGISU:123021300231-8. Research was performed using the equipment of the Resource Centre \u2018GeoModel\u2019, Interdisciplinary Resource Centre for Nanotechnology, Centre for Diagnostics of Functional Materials for Medicine, Pharmacology and Nanoelectronics, Magnetic Resonance Research Centre, Centre for Physical Methods of Surface Investigation; computational resources provided by Resource Centre \u2018Computer Centre of SPbU\u2019 and Centre for Chemical Analysis and Materials Research of the Research Park of Saint Petersburg State University.

PY - 2024/9/20

Y1 - 2024/9/20

N2 - The article is devoted to synthesis, characterisation and complex investigation of biocompatibility and cytotoxicity of graphene oxide conjugate with folic acid and cytarabine cytostatic (GO-FA-Cyt). GO was synthesised using a patented modified Hummers and Offeman's method leading to the production of GO enriched with oxygen-containing functional groups (⁓85%). Obtained nanomaterial was covalently modified with FA and non-covalently functionalised with Cyt and characterised using 13C NMR, Raman, and IR spectroscopy, XRD and DLS for aqueous dispersions. Biocompatibility and biological activity studies included investigation of haemocompatibility, antioxidant activity, genotoxicity, and cytotoxicity towards Jurkat, HL60 cell lines. The kinetic dependence of the adsorption of Cyt on the covalent GO-FA conjugate was obtained and described using various models: Brunauer, Emmett and Teller, Langmuir. Experimental data on release were fitted using zero-order, first-order and Korsmeyer–Peppas models. © 2024

AB - The article is devoted to synthesis, characterisation and complex investigation of biocompatibility and cytotoxicity of graphene oxide conjugate with folic acid and cytarabine cytostatic (GO-FA-Cyt). GO was synthesised using a patented modified Hummers and Offeman's method leading to the production of GO enriched with oxygen-containing functional groups (⁓85%). Obtained nanomaterial was covalently modified with FA and non-covalently functionalised with Cyt and characterised using 13C NMR, Raman, and IR spectroscopy, XRD and DLS for aqueous dispersions. Biocompatibility and biological activity studies included investigation of haemocompatibility, antioxidant activity, genotoxicity, and cytotoxicity towards Jurkat, HL60 cell lines. The kinetic dependence of the adsorption of Cyt on the covalent GO-FA conjugate was obtained and described using various models: Brunauer, Emmett and Teller, Langmuir. Experimental data on release were fitted using zero-order, first-order and Korsmeyer–Peppas models. © 2024

KW - Biocompatibility

KW - Cytarabine

KW - Cytotoxicity

KW - Folic acid

KW - Graphene oxide

KW - Bioactivity

KW - Cell culture

KW - Graphene

KW - Organic acids

KW - Cytostatic

KW - Folic acids

KW - Functionalized

KW - Graphene oxides

KW - IR-spectroscopy

KW - NMR-spectroscopy

KW - Oxygen-containing functional groups

KW - Synthesised

KW - XRD

KW - collagen

KW - cytarabine

KW - folate receptor

KW - folic acid

KW - graphene oxide

KW - adsorption

KW - antioxidant activity

KW - Article

KW - binding competition

KW - binding site

KW - biocompatibility

KW - biological activity

KW - carbon nuclear magnetic resonance

KW - controlled study

KW - drug cytotoxicity

KW - drug release

KW - drug synthesis

KW - fluorescence microscopy

KW - genotoxicity

KW - HL-60 cell line

KW - human

KW - human cell

KW - infrared spectroscopy

KW - Jurkat cell line

KW - Raman spectrometry

KW - ultraviolet visible spectroscopy

KW - X ray diffraction

KW - zeta potential

UR - https://www.mendeley.com/catalogue/d272bc27-3c9f-3256-8f39-d54b8e174786/

U2 - 10.1016/j.colsurfa.2024.134360

DO - 10.1016/j.colsurfa.2024.134360

M3 - статья

VL - 697

JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects

JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects

SN - 0927-7757

M1 - 134360

ER -