TY - JOUR

T1 - Symbiotic bacteria of the gall-inducing mite Fragariocoptes setiger (Eriophyoidea) and phylogenomic resolution of the eriophyoid position among Acari

AU - Klimov, Pavel B.

AU - Chetverikov, Philipp E.

AU - Dodueva, Irina E.

AU - Vishnyakov, Andrey E.

AU - Bolton, Samuel J.

AU - Paponova, Svetlana S.

AU - Lutova, Ljudmila A.

AU - Tolstikov, Andrey V.

N1 - Klimov, P.B., Chetverikov, P.E., Dodueva, I.E. et al. Symbiotic bacteria of the gall-inducing mite Fragariocoptes setiger (Eriophyoidea) and phylogenomic resolution of the eriophyoid position among Acari. Sci Rep 12, 3811 (2022). https://doi.org/10.1038/s41598-022-07535-3

PY - 2022/12/1

Y1 - 2022/12/1

N2 - Eriophyoid mites represent a hyperdiverse, phytophagous lineage with an unclear phylogenetic position. These mites have succeeded in colonizing nearly every seed plant species, and this evolutionary success was in part due to the mites' ability to induce galls in plants. A gall is a unique niche that provides the inducer of this modification with vital resources. The exact mechanism of gall formation is still not understood, even as to whether it is endogenic (mites directly cause galls) or exogenic (symbiotic microorganisms are involved). Here we (i) investigate the phylogenetic affinities of eriophyoids and (ii) use comparative metagenomics to test the hypothesis that the endosymbionts of eriophyoid mites are involved in gall formation. Our phylogenomic analysis robustly inferred eriophyoids as closely related to Nematalycidae, a group of deep-soil mites belonging to Endeostigmata. Our comparative metagenomics, fluorescence in situ hybridization, and electron microscopy experiments identified two candidate endosymbiotic bacteria shared across samples, however, it is unlikely that they are gall inducers (morphotype1: novel Wolbachia, morphotype2: possibly Agrobacterium tumefaciens). We also detected an array of plant pathogens associated with galls that may be vectored by the mites, and we determined a mite pathogenic virus (Betabaculovirus) that could be tested for using in biocontrol of agricultural pest mites.

AB - Eriophyoid mites represent a hyperdiverse, phytophagous lineage with an unclear phylogenetic position. These mites have succeeded in colonizing nearly every seed plant species, and this evolutionary success was in part due to the mites' ability to induce galls in plants. A gall is a unique niche that provides the inducer of this modification with vital resources. The exact mechanism of gall formation is still not understood, even as to whether it is endogenic (mites directly cause galls) or exogenic (symbiotic microorganisms are involved). Here we (i) investigate the phylogenetic affinities of eriophyoids and (ii) use comparative metagenomics to test the hypothesis that the endosymbionts of eriophyoid mites are involved in gall formation. Our phylogenomic analysis robustly inferred eriophyoids as closely related to Nematalycidae, a group of deep-soil mites belonging to Endeostigmata. Our comparative metagenomics, fluorescence in situ hybridization, and electron microscopy experiments identified two candidate endosymbiotic bacteria shared across samples, however, it is unlikely that they are gall inducers (morphotype1: novel Wolbachia, morphotype2: possibly Agrobacterium tumefaciens). We also detected an array of plant pathogens associated with galls that may be vectored by the mites, and we determined a mite pathogenic virus (Betabaculovirus) that could be tested for using in biocontrol of agricultural pest mites.

KW - Animals

KW - Bacteria

KW - Biological Evolution

KW - In Situ Hybridization, Fluorescence

KW - Mites/genetics

KW - Phylogeny

KW - Plants

KW - GEN-NOV

KW - ACARIFORMES

KW - LINEAGES

KW - EFFECTORS

KW - GREEN-ISLAND PHENOTYPE

KW - PARASITISM

KW - CYTOKININS

KW - INSECTS

KW - WOLBACHIA

KW - PLANT MANIPULATION

UR - http://www.scopus.com/inward/record.url?scp=85126080222&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/f6c46a24-cadd-3878-9d40-415138d3129f/

U2 - 10.1038/s41598-022-07535-3

DO - 10.1038/s41598-022-07535-3

M3 - Article

C2 - 35264574

AN - SCOPUS:85126080222

VL - 12

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 3811

ER -