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STXBP1 forms amyloid-like aggregates in rat brain and demonstrates amyloid properties in bacterial expression system. / Чиринскайте, Ангелина Валерьевна; Задорский, Сергей Павлович; Сопова, Юлия Викторовна; Белашова, Татьяна Алексеевна; Велижанина, Мария Евгеньевна; Синюкова, Вера.

в: Prion, Том 15, № 1, 2021, стр. 29-36.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

Harvard

Чиринскайте, АВ, Задорский, СП, Сопова, ЮВ, Белашова, ТА, Велижанина, МЕ & Синюкова, В 2021, 'STXBP1 forms amyloid-like aggregates in rat brain and demonstrates amyloid properties in bacterial expression system', Prion, Том. 15, № 1, стр. 29-36. https://doi.org/10.1080/19336896.2021.1883980

APA

Чиринскайте, А. В., Задорский, С. П., Сопова, Ю. В., Белашова, Т. А., Велижанина, М. Е., & Синюкова, В. (2021). STXBP1 forms amyloid-like aggregates in rat brain and demonstrates amyloid properties in bacterial expression system. Prion, 15(1), 29-36. https://doi.org/10.1080/19336896.2021.1883980

Vancouver

Чиринскайте АВ, Задорский СП, Сопова ЮВ, Белашова ТА, Велижанина МЕ, Синюкова В. STXBP1 forms amyloid-like aggregates in rat brain and demonstrates amyloid properties in bacterial expression system. Prion. 2021;15(1):29-36. https://doi.org/10.1080/19336896.2021.1883980

Author

Чиринскайте, Ангелина Валерьевна ; Задорский, Сергей Павлович ; Сопова, Юлия Викторовна ; Белашова, Татьяна Алексеевна ; Велижанина, Мария Евгеньевна ; Синюкова, Вера. / STXBP1 forms amyloid-like aggregates in rat brain and demonstrates amyloid properties in bacterial expression system. в: Prion. 2021 ; Том 15, № 1. стр. 29-36.

BibTeX

@article{73429df3926b492ab932760d4a8a1058,
title = "STXBP1 forms amyloid-like aggregates in rat brain and demonstrates amyloid properties in bacterial expression system",
abstract = "Amyloids are the fibrillar protein aggregates with cross-β structure. Traditionally amyloids were associated with pathology, however, nowadays more data is emerging about functional amyloids playing essential roles in cellular processes. We conducted screening for functional amyloids in rat brain. One of the identified proteins was STXBP1 taking part in vesicular transport and neurotransmitter secretion. Using SDD-AGE and protein fractionation we found out that STXBP1 forms small detergent-insoluble aggregates in rat brain. With immunoprecipitation analysis and C-DAG system, we showed that STXBP1 forms amyloid-like fibrils. Thus, STXBP1 demonstrates amyloid properties in rat brain and in bacterial expression system.",
keywords = "амилоиды, функциональные амилоиды, крыса, SNARE, Amyloid, Functional amyloid, Rat, Munc18-1, SNARE, STXBP1, Proteomic screening, amyloid, rat, brain, functional amyloid, Rats, Amyloid/metabolism, Munc18 Proteins/metabolism, Animals, Amyloidosis, Brain/metabolism",
author = "Чиринскайте, {Ангелина Валерьевна} and Задорский, {Сергей Павлович} and Сопова, {Юлия Викторовна} and Белашова, {Татьяна Алексеевна} and Велижанина, {Мария Евгеньевна} and Вера Синюкова",
note = "Publisher Copyright: {\textcopyright} 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.",
year = "2021",
doi = "10.1080/19336896.2021.1883980",
language = "English",
volume = "15",
pages = "29--36",
journal = "Prion",
issn = "1933-6896",
publisher = "Landes Bioscience",
number = "1",

}

RIS

TY - JOUR

T1 - STXBP1 forms amyloid-like aggregates in rat brain and demonstrates amyloid properties in bacterial expression system

AU - Чиринскайте, Ангелина Валерьевна

AU - Задорский, Сергей Павлович

AU - Сопова, Юлия Викторовна

AU - Белашова, Татьяна Алексеевна

AU - Велижанина, Мария Евгеньевна

AU - Синюкова, Вера

N1 - Publisher Copyright: © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

PY - 2021

Y1 - 2021

N2 - Amyloids are the fibrillar protein aggregates with cross-β structure. Traditionally amyloids were associated with pathology, however, nowadays more data is emerging about functional amyloids playing essential roles in cellular processes. We conducted screening for functional amyloids in rat brain. One of the identified proteins was STXBP1 taking part in vesicular transport and neurotransmitter secretion. Using SDD-AGE and protein fractionation we found out that STXBP1 forms small detergent-insoluble aggregates in rat brain. With immunoprecipitation analysis and C-DAG system, we showed that STXBP1 forms amyloid-like fibrils. Thus, STXBP1 demonstrates amyloid properties in rat brain and in bacterial expression system.

AB - Amyloids are the fibrillar protein aggregates with cross-β structure. Traditionally amyloids were associated with pathology, however, nowadays more data is emerging about functional amyloids playing essential roles in cellular processes. We conducted screening for functional amyloids in rat brain. One of the identified proteins was STXBP1 taking part in vesicular transport and neurotransmitter secretion. Using SDD-AGE and protein fractionation we found out that STXBP1 forms small detergent-insoluble aggregates in rat brain. With immunoprecipitation analysis and C-DAG system, we showed that STXBP1 forms amyloid-like fibrils. Thus, STXBP1 demonstrates amyloid properties in rat brain and in bacterial expression system.

KW - амилоиды

KW - функциональные амилоиды

KW - крыса

KW - SNARE

KW - Amyloid

KW - Functional amyloid

KW - Rat

KW - Munc18-1

KW - SNARE

KW - STXBP1

KW - Proteomic screening

KW - amyloid

KW - rat

KW - brain

KW - functional amyloid

KW - Rats

KW - Amyloid/metabolism

KW - Munc18 Proteins/metabolism

KW - Animals

KW - Amyloidosis

KW - Brain/metabolism

UR - http://www.scopus.com/inward/record.url?scp=85101075836&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/cb407e29-2c8a-31c0-9f87-984e49702fdf/

U2 - 10.1080/19336896.2021.1883980

DO - 10.1080/19336896.2021.1883980

M3 - Article

C2 - 33590815

VL - 15

SP - 29

EP - 36

JO - Prion

JF - Prion

SN - 1933-6896

IS - 1

ER -

ID: 74447381