Today, the study of ion transport in nanochannels is important not only in the field of fundamental science, but also in various practical applications such as biomedicine [1], genetic engineering [2], sensor development etc. The use of lithography methods opens up wide possibilities for the creation of micro- and nanochannels, nanoscale pores, as well as the implantation of functional active nanostructures into them. In this study, we developed and tested a technique for fabrication of microfluidic chips with two flow cells (cameras) with 0.05 cm3 volume, interconnected with a system of nanochannels (width 90 nm, depth 20 nm). For nanochannels fabrication and study of the FIB etching, CrossBeam Neon40 (Carl Zeiss) system of crossed electron and ion beams was used. This technique allows to create arrays of channels with various widths and depths. To study the transport properties of the obtained nanochannels, an experimental setup was developed and assembled, the main element of which is a microfiluidic chip, consisting of two half-volumes separated by nanochannels. Ag / AgCl wires were used as measuring electrodes. A series of experiments was carried out to record the current-voltage (I-V) characteristics of the nanochannel at various electrolyte concentrations. An aqueous KCl solution was chosen as the base electrolyte. The I – V characteristic was measured in the voltage range of -0.2 ... + 0.2V. With an increase in concentration from 0.01 to 1 M, the channel conductivity increased from 7.3 to 11.8 pS. The authors attributed such a low increase in conductivity to the presence of a significant uncompensated electric charge within the channels. The synthesized systems with micro- and nanoscale and nanopores can be used in studies of the transport properties of various ions and molecules. In addition, such structures can be successfully used in the development of highly sensitive biosensor systems and in lab-on-a-chip systems.