TY - JOUR

T1 - Structural-functional organization of the cells of Brc-1 mutant of Chlamydomonas reinhardtii accumulating protoporphyrin IX in the dark

AU - Ladygin, V. G.

AU - Chekunova, E. M.

AU - Semenova, G. A.

AU - Kosobryukhov, A. A.

PY - 2014

Y1 - 2014

N2 - The structural-functional characteristics of the cells of wild type CC-124 and Brc-1 mutant of the unicellular green algae Chlamydomonas reinhardtii grown in the dark and in the light were studied. The cells of the wild type in heterotrophic and mixotrophic conditions had a well developed structure and high functional activity due to the ability of the cells to synthesize chlorophyll both in the light and in the dark. The cells of Brc-1 mutant lost their ability to synthesize chlorophyll in the dark and the cell color was orange due to brc-1 mutation in the nuclear gene LTS3 that regulated the activity of Mg-chelatase enzyme. In the dark the mutant cells accumulated protoporphyrin IX and had weakly developed structure with low functional activity. Because of the high content of protoporphyrin IX, even a short-term exposure of the Brc-1 mutant cells to the light was accompanied by very strong destructive changes in all the membranes in a cell: plasmalemma, chloroplast, mitochondrion, envelopes of the nucleus a

AB - The structural-functional characteristics of the cells of wild type CC-124 and Brc-1 mutant of the unicellular green algae Chlamydomonas reinhardtii grown in the dark and in the light were studied. The cells of the wild type in heterotrophic and mixotrophic conditions had a well developed structure and high functional activity due to the ability of the cells to synthesize chlorophyll both in the light and in the dark. The cells of Brc-1 mutant lost their ability to synthesize chlorophyll in the dark and the cell color was orange due to brc-1 mutation in the nuclear gene LTS3 that regulated the activity of Mg-chelatase enzyme. In the dark the mutant cells accumulated protoporphyrin IX and had weakly developed structure with low functional activity. Because of the high content of protoporphyrin IX, even a short-term exposure of the Brc-1 mutant cells to the light was accompanied by very strong destructive changes in all the membranes in a cell: plasmalemma, chloroplast, mitochondrion, envelopes of the nucleus a

KW - Brc-1 mutant

KW - Mg-chelatase

KW - chlorophyll

KW - protoporphyrin IX

KW - Mg-protoporphyrin IX

KW - ultrastructure of membranes

KW - O2-gas exchange

U2 - 10.1134/S0006350914040162

DO - 10.1134/S0006350914040162

M3 - Article

VL - 59

SP - 568

EP - 576

JO - Biophysics (Russian Federation)

JF - Biophysics (Russian Federation)

SN - 0006-3509

IS - 4

ER -