Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Strain-Specific Features of Primary Metabolome Characteristic for Extremotolerant/Extremophilic Cyanobacteria Under Long-Term Storage. / Билова, Татьяна Евгеньевна; Голушко, Никита Игоревич; Фролова, Надежда Владимировна; Соболева, Алена; Силинская, Светлана Александровна; Хакулова, Анна Андреевна; Орлова, Анастасия ; Синетова, Мария; Лось, Дмитрий Анатольевич; Фролов, Андрей.
в: International Journal of Molecular Sciences, Том 26, № 5, 2201, 28.02.2025.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Strain-Specific Features of Primary Metabolome Characteristic for Extremotolerant/Extremophilic Cyanobacteria Under Long-Term Storage
AU - Билова, Татьяна Евгеньевна
AU - Голушко, Никита Игоревич
AU - Фролова, Надежда Владимировна
AU - Соболева, Алена
AU - Силинская, Светлана Александровна
AU - Хакулова, Анна Андреевна
AU - Орлова, Анастасия
AU - Синетова, Мария
AU - Лось, Дмитрий Анатольевич
AU - Фролов, Андрей
PY - 2025/2/28
Y1 - 2025/2/28
N2 - Cyanobacteria isolated from extreme habitats are promising in biotechnology due to their high adaptability to unfavorable environments and their specific natural products. Therefore, these organisms are stored under a reduced light supply in multiple collections worldwide. However, it remains unclear whether these strains maintain constitutively expressed primary metabolome features associated with their unique adaptations. To address this question, a comparative analysis of primary metabolomes of twelve cyanobacterial strains from diverse extreme habitats was performed by a combined GC-MS/LC-MS approach. The results revealed that all these cyanobacterial strains exhibited clear differences in their patterns of primary metabolites. These metabolic differences were more pronounced for the strains originating from ecologically different extreme environments. Extremotolerant terrestrial and freshwater strains contained lower strain-specifically accumulated primary metabolites than extremophilic species from habitats with high salinity and alkalinity. The latter group of strains was highly diverse in amounts of specific primary metabolites. This might indicate essentially different molecular mechanisms and metabolic pathways behind the survival of the microorganisms in saline and alkaline environments. The identified strain-specific metabolites are discussed with respect to the metabolic processes that might impact maintaining the viability of cyanobacteria during their storage and indicate unique adaptations formed in their original extreme habitats.
AB - Cyanobacteria isolated from extreme habitats are promising in biotechnology due to their high adaptability to unfavorable environments and their specific natural products. Therefore, these organisms are stored under a reduced light supply in multiple collections worldwide. However, it remains unclear whether these strains maintain constitutively expressed primary metabolome features associated with their unique adaptations. To address this question, a comparative analysis of primary metabolomes of twelve cyanobacterial strains from diverse extreme habitats was performed by a combined GC-MS/LC-MS approach. The results revealed that all these cyanobacterial strains exhibited clear differences in their patterns of primary metabolites. These metabolic differences were more pronounced for the strains originating from ecologically different extreme environments. Extremotolerant terrestrial and freshwater strains contained lower strain-specifically accumulated primary metabolites than extremophilic species from habitats with high salinity and alkalinity. The latter group of strains was highly diverse in amounts of specific primary metabolites. This might indicate essentially different molecular mechanisms and metabolic pathways behind the survival of the microorganisms in saline and alkaline environments. The identified strain-specific metabolites are discussed with respect to the metabolic processes that might impact maintaining the viability of cyanobacteria during their storage and indicate unique adaptations formed in their original extreme habitats.
KW - Adaptation, Physiological
KW - Cyanobacteria/metabolism
KW - Ecosystem
KW - Extremophiles/metabolism
KW - Gas Chromatography-Mass Spectrometry
KW - Metabolome
KW - Metabolomics/methods
KW - Salinity
KW - Species Specificity
KW - primary metabolites
KW - adaptation to extreme conditions
KW - GC-MS
KW - LC-MS
KW - haloalkaliphiles and natronophiles
KW - metabolomics
KW - extremophiles
KW - cyanobacterial strains
KW - metabolic pathways
UR - https://www.mendeley.com/catalogue/812f554a-c97a-349b-96eb-d21b37350b33/
UR - https://elibrary.ru/item.asp?id=81597739
U2 - 10.3390/ijms26052201
DO - 10.3390/ijms26052201
M3 - Article
C2 - 40076823
VL - 26
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1422-0067
IS - 5
M1 - 2201
ER -
ID: 133774727