Standard

Sozdanie "iskusstvennykh gibridnykh operonov s chastichno perekryvaiushchimisia genami" dlia dostizheniia ékspressii geterologichnykh genov v kletkakh Escherichia coli. / Mashko, S. V.; Lapidus, A. L.; Trukhan, M. E.; Podkovyrov, S. M.

в: Molekulyarnaya Biologiya, Том 21, № 5, 09.1987, стр. 1297-1309.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

Harvard

Mashko, SV, Lapidus, AL, Trukhan, ME & Podkovyrov, SM 1987, 'Sozdanie "iskusstvennykh gibridnykh operonov s chastichno perekryvaiushchimisia genami" dlia dostizheniia ékspressii geterologichnykh genov v kletkakh Escherichia coli.', Molekulyarnaya Biologiya, Том. 21, № 5, стр. 1297-1309.

APA

Mashko, S. V., Lapidus, A. L., Trukhan, M. E., & Podkovyrov, S. M. (1987). Sozdanie "iskusstvennykh gibridnykh operonov s chastichno perekryvaiushchimisia genami" dlia dostizheniia ékspressii geterologichnykh genov v kletkakh Escherichia coli. Molekulyarnaya Biologiya, 21(5), 1297-1309.

Vancouver

Mashko SV, Lapidus AL, Trukhan ME, Podkovyrov SM. Sozdanie "iskusstvennykh gibridnykh operonov s chastichno perekryvaiushchimisia genami" dlia dostizheniia ékspressii geterologichnykh genov v kletkakh Escherichia coli. Molekulyarnaya Biologiya. 1987 Сент.;21(5):1297-1309.

Author

Mashko, S. V. ; Lapidus, A. L. ; Trukhan, M. E. ; Podkovyrov, S. M. / Sozdanie "iskusstvennykh gibridnykh operonov s chastichno perekryvaiushchimisia genami" dlia dostizheniia ékspressii geterologichnykh genov v kletkakh Escherichia coli. в: Molekulyarnaya Biologiya. 1987 ; Том 21, № 5. стр. 1297-1309.

BibTeX

@article{190d43c486ce4b679bd942ef46875a24,
title = "Sozdanie {"}iskusstvennykh gibridnykh operonov s chastichno perekryvaiushchimisia genami{"} dlia dostizheniia {\'e}kspressii geterologichnykh genov v kletkakh Escherichia coli.",
abstract = "A new method of optimization of foreign gene expression in E. coli, based on the construction of hybrid operons with partially overlapping genes is described. The partial overlapping of the translation termination and initiation sites in the formed operon must provide translational coupling of appropriate gene product synthesis. Such an approach has provided the synthesis of human interferon alpha F in E. coli cells under the control of the lacUV5-promotor up to about (3-4).10(7) units per liter of bacterial culture. The reinitiation of the distal gene translation is shown to take place in the intercistronic region. Substitution of the lacUV5 promotor by the more efficient tac one allowed to increase the synthesis level of interferon alpha F to (1-2).10(8) units per liter. The conclusion is made about the equimolarity of distal and proximal to the promotor genes products syntheses when the intercistronic region of E. coli trpE-trpD genes are used for translational coupling.",
author = "Mashko, {S. V.} and Lapidus, {A. L.} and Trukhan, {M. E.} and Podkovyrov, {S. M.}",
year = "1987",
month = sep,
language = "русский",
volume = "21",
pages = "1297--1309",
journal = "МОЛЕКУЛЯРНАЯ БИОЛОГИЯ",
issn = "0026-8984",
publisher = "Российская академия наук",
number = "5",

}

RIS

TY - JOUR

T1 - Sozdanie "iskusstvennykh gibridnykh operonov s chastichno perekryvaiushchimisia genami" dlia dostizheniia ékspressii geterologichnykh genov v kletkakh Escherichia coli.

AU - Mashko, S. V.

AU - Lapidus, A. L.

AU - Trukhan, M. E.

AU - Podkovyrov, S. M.

PY - 1987/9

Y1 - 1987/9

N2 - A new method of optimization of foreign gene expression in E. coli, based on the construction of hybrid operons with partially overlapping genes is described. The partial overlapping of the translation termination and initiation sites in the formed operon must provide translational coupling of appropriate gene product synthesis. Such an approach has provided the synthesis of human interferon alpha F in E. coli cells under the control of the lacUV5-promotor up to about (3-4).10(7) units per liter of bacterial culture. The reinitiation of the distal gene translation is shown to take place in the intercistronic region. Substitution of the lacUV5 promotor by the more efficient tac one allowed to increase the synthesis level of interferon alpha F to (1-2).10(8) units per liter. The conclusion is made about the equimolarity of distal and proximal to the promotor genes products syntheses when the intercistronic region of E. coli trpE-trpD genes are used for translational coupling.

AB - A new method of optimization of foreign gene expression in E. coli, based on the construction of hybrid operons with partially overlapping genes is described. The partial overlapping of the translation termination and initiation sites in the formed operon must provide translational coupling of appropriate gene product synthesis. Such an approach has provided the synthesis of human interferon alpha F in E. coli cells under the control of the lacUV5-promotor up to about (3-4).10(7) units per liter of bacterial culture. The reinitiation of the distal gene translation is shown to take place in the intercistronic region. Substitution of the lacUV5 promotor by the more efficient tac one allowed to increase the synthesis level of interferon alpha F to (1-2).10(8) units per liter. The conclusion is made about the equimolarity of distal and proximal to the promotor genes products syntheses when the intercistronic region of E. coli trpE-trpD genes are used for translational coupling.

UR - http://www.scopus.com/inward/record.url?scp=0023403239&partnerID=8YFLogxK

M3 - статья

C2 - 3316974

AN - SCOPUS:0023403239

VL - 21

SP - 1297

EP - 1309

JO - МОЛЕКУЛЯРНАЯ БИОЛОГИЯ

JF - МОЛЕКУЛЯРНАЯ БИОЛОГИЯ

SN - 0026-8984

IS - 5

ER -

ID: 90141650