Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Soluble guanylate cyclase as the key enzyme in the modulating effect of NO on metabotropic glutamate receptors. / Ryzhova, I. V.; Nozdrachev, A. D.; Tobias, T. V.; Vershinina, E. A.
в: Acta Naturae, Том 10, № 2, 01.01.2018, стр. 71-78.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Soluble guanylate cyclase as the key enzyme in the modulating effect of NO on metabotropic glutamate receptors
AU - Ryzhova, I. V.
AU - Nozdrachev, A. D.
AU - Tobias, T. V.
AU - Vershinina, E. A.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - The synaptic plasticity of the afferent synapse of the vestibular apparatus is defined by the dynamic interaction of ionotropic and metabotropic glutamate receptors and the modulators of synaptic transmission. It was shown that nitric oxide modulates iGluR responses. In this paper, the effect of NO on the function of the afferent synapse mGluR was investigated. Inhibitor of nitric oxide synthase lowered the level of background activity but increased the amplitude of the responses of groups I and II mGluR agonist ACPD. Donor NO SNAP increased the level of background activity. Short-term perfusion of the synaptic region with low concentrations of SNAP led to a decrease in the amplitude of the answers of mGluR agonists ACPD and DHPG. The inhibitory effect of the NO donor was eliminated under blockade of soluble guanylate cyclase with a specific inhibitor ODQ. A prolonged application of NO did not cause a statistically significant change in the amplitude of the ACPD response. However, SNAP at concentrations of 10 and 100 μM increased the amplitude of the mGluR agonist responses 30 and 15 minutes, respectively, after termination of the NO donor exposure. The obtained data show the multidirectional effect of NO on the function of mGluR and testify to the existence of a complex modulating mechanism of the afferent flow from vestibular organs to the central nervous system.
AB - The synaptic plasticity of the afferent synapse of the vestibular apparatus is defined by the dynamic interaction of ionotropic and metabotropic glutamate receptors and the modulators of synaptic transmission. It was shown that nitric oxide modulates iGluR responses. In this paper, the effect of NO on the function of the afferent synapse mGluR was investigated. Inhibitor of nitric oxide synthase lowered the level of background activity but increased the amplitude of the responses of groups I and II mGluR agonist ACPD. Donor NO SNAP increased the level of background activity. Short-term perfusion of the synaptic region with low concentrations of SNAP led to a decrease in the amplitude of the answers of mGluR agonists ACPD and DHPG. The inhibitory effect of the NO donor was eliminated under blockade of soluble guanylate cyclase with a specific inhibitor ODQ. A prolonged application of NO did not cause a statistically significant change in the amplitude of the ACPD response. However, SNAP at concentrations of 10 and 100 μM increased the amplitude of the mGluR agonist responses 30 and 15 minutes, respectively, after termination of the NO donor exposure. The obtained data show the multidirectional effect of NO on the function of mGluR and testify to the existence of a complex modulating mechanism of the afferent flow from vestibular organs to the central nervous system.
KW - Metabotropic glutamate receptors
KW - Nitric oxide
KW - Soluble guanylate cyclase
KW - Synaptic plasticity
KW - Vestibular apparatus
UR - http://www.scopus.com/inward/record.url?scp=85054098228&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85054098228
VL - 10
SP - 71
EP - 78
JO - Acta Naturae
JF - Acta Naturae
SN - 2075-8251
IS - 2
ER -
ID: 37013197