Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Single-cell transcriptomics of human embryos identifies multiple sympathoblast lineages with potential implications for neuroblastoma origin. / Kameneva, P.; Artemov, A.V.; Kastriti, Maria Eleni ; Faure, L.; Olsen, T.K.; Otte, J.; Erickson, A.; Semsch, B.; Andersson, E.R.; Ratz, M.; Frisén, Jonas; Tischler, A.S.; de Krijger, R.R.; Bouderlique, T.; Akkuratova, N.; Vorontsova, M.; Gusev, O.; Fried, Kaj; Sundstrom, E.; Mei, S.; Kogner, P.; Baryawno, N.; Kharchenko, P.V.; Adameyko, Igor.
в: Nature Genetics, Том 53, № 5, 05.2021, стр. 694-706.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Single-cell transcriptomics of human embryos identifies multiple sympathoblast lineages with potential implications for neuroblastoma origin
AU - Kameneva, P.
AU - Artemov, A.V.
AU - Kastriti, Maria Eleni
AU - Faure, L.
AU - Olsen, T.K.
AU - Otte, J.
AU - Erickson, A.
AU - Semsch, B.
AU - Andersson, E.R.
AU - Ratz, M.
AU - Frisén, Jonas
AU - Tischler, A.S.
AU - de Krijger, R.R.
AU - Bouderlique, T.
AU - Akkuratova, N.
AU - Vorontsova, M.
AU - Gusev, O.
AU - Fried, Kaj
AU - Sundstrom, E.
AU - Mei, S.
AU - Kogner, P.
AU - Baryawno, N.
AU - Kharchenko, P.V.
AU - Adameyko, Igor
N1 - Publisher Copyright: © 2021, The Author(s), under exclusive licence to Springer Nature America, Inc.
PY - 2021/5
Y1 - 2021/5
N2 - Characterization of the progression of cellular states during human embryogenesis can provide insights into the origin of pediatric diseases. We examined the transcriptional states of neural crest– and mesoderm-derived lineages differentiating into adrenal glands, kidneys, endothelium and hematopoietic tissue between post-conception weeks 6 and 14 of human development. Our results reveal transitions connecting the intermediate mesoderm and progenitors of organ primordia, the hematopoietic system and endothelial subtypes. Unexpectedly, by using a combination of single-cell transcriptomics and lineage tracing, we found that intra-adrenal sympathoblasts at that stage are directly derived from nerve-associated Schwann cell precursors, similarly to local chromaffin cells, whereas the majority of extra-adrenal sympathoblasts arise from the migratory neural crest. In humans, this process persists during several weeks of development within the large intra-adrenal ganglia-like structures, which may also serve as reservoirs of originating cells in neuroblastoma.
AB - Characterization of the progression of cellular states during human embryogenesis can provide insights into the origin of pediatric diseases. We examined the transcriptional states of neural crest– and mesoderm-derived lineages differentiating into adrenal glands, kidneys, endothelium and hematopoietic tissue between post-conception weeks 6 and 14 of human development. Our results reveal transitions connecting the intermediate mesoderm and progenitors of organ primordia, the hematopoietic system and endothelial subtypes. Unexpectedly, by using a combination of single-cell transcriptomics and lineage tracing, we found that intra-adrenal sympathoblasts at that stage are directly derived from nerve-associated Schwann cell precursors, similarly to local chromaffin cells, whereas the majority of extra-adrenal sympathoblasts arise from the migratory neural crest. In humans, this process persists during several weeks of development within the large intra-adrenal ganglia-like structures, which may also serve as reservoirs of originating cells in neuroblastoma.
KW - Cancer
KW - Computational biology and bioinformatics
KW - Developmental biology
KW - Embryonal neoplasms
KW - Genetics
KW - Schwann Cells/metabolism
KW - Sympathoadrenal System/embryology
KW - Chromaffin Cells/metabolism
KW - Humans
KW - Gene Expression Regulation, Neoplastic
KW - Tumor Microenvironment
KW - Infant
KW - Embryonic Development
KW - Neuroblastoma/embryology
KW - Transcriptome/genetics
KW - Embryo, Mammalian/metabolism
KW - Cell Lineage
KW - Animals
KW - Neural Stem Cells/metabolism
KW - Gene Expression Regulation, Developmental
KW - Mice
KW - Single-Cell Analysis
KW - Cluster Analysis
UR - http://www.scopus.com/inward/record.url?scp=85104053714&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/4274f8a9-0eda-3aa4-a1e9-ae23c601cee9/
U2 - 10.1038/s41588-021-00818-x
DO - 10.1038/s41588-021-00818-x
M3 - Article
C2 - 33833454
VL - 53
SP - 694
EP - 706
JO - Nature Genetics
JF - Nature Genetics
SN - 1061-4036
IS - 5
ER -
ID: 87436037