TY - JOUR

T1 - Sewing up the wounds : The epithelial morphogenesis as a central mechanism of calcaronean sponge regeneration

AU - Лавров, Андрей Игоревич

AU - Большаков, Федор Васильевич

AU - Tokina, D.B.

AU - Ересковский, Александр Вадимович

N1 - Funding Information: Russian Foundation for Basic Research, Grant/ Award Number: 16‐04‐00084; Russian Science Foundation, Grant/Award Number: 17‐14‐01089; A*MIDEX, Grant/Award Number: ANR‐11‐LABX‐0061; Excellence Initiative of Aix‐Marseille University— A*MIDEX Funding Information: Authors gratefully thank Fernanda Azevedo, Universidade Federal do Rio de Janeiro and Irene Ekimova, Moscow State University for helping with the species determination, Philippe Willenz for help with design of the calcein experiments, Alexandre Altié of Service Commun de Microscopie Électronique et Photo-graphie Faculté de Médecine La Timone, Aix‐Marseille Université, the Electron Microscopy Laboratory of the Shared Facilities Center of Lomonosov Moscow State University sponsored by the RF Ministry of Education and Science and Research Resource Center for Molecular and Cell Technologies at St. Petersburg State University, the staff of the Common Service of morphology in IMBE—for assistance with electron and confocal microscopy studies. The light microscopy study was conducted using equipment of the Center of microscopy WSBS MSU. This work was supported by grants of Russian Foundation for Basic Research no. 16‐04‐00084, the Russian Science Foundation no. 17‐14‐01089 (histological and ultrastructural studies). This work also is a contribution to Labex OT‐Med (n_ ANR‐11‐LABX‐0061) and has received funding from Excellence Initiative of Aix‐Marseille University—A*MIDEX, a French ’’Investissements d’Avenir’’ program for travel expenses.

PY - 2018/9/1

Y1 - 2018/9/1

N2 - Sponges (Porifera) demonstrate prominent regeneration abilities and possess a wide variety of mechanisms, used during this process. In the current study, we combined in vivo observations with histological, immunohistochemical, and ultrastructural technics to elucidate the fine cellular mechanisms of the regeneration in the calcareous sponge Leucosolenia cf. variabilis. The regeneration of Leucosolenia cf. variabilis ends within 4–6 days. The crucial step of the process is the formation of the transient regenerative membrane, formed by the epithelial morphogenesis—spreading of the intact exopinacoderm and choanoderm. The spreading of the choanoderm is accompanied by the transdifferentiation of the choanocytes. The regenerative membrane develops without any contribution of the mesohyl cells. Subsequently, the membrane gradually transforms into the body wall. The cell proliferation is neither affected nor contributes to the regeneration at any stage. Thus, Leucosolenia cf. variabilis regeneration relies on the remodeling of the intact tissues through the epithelial morphogenesis, accompanied by the transdifferentiation of some differentiated cell types, which makes it similar to the regeneration in homoscleromorphs and eumetazoans.

AB - Sponges (Porifera) demonstrate prominent regeneration abilities and possess a wide variety of mechanisms, used during this process. In the current study, we combined in vivo observations with histological, immunohistochemical, and ultrastructural technics to elucidate the fine cellular mechanisms of the regeneration in the calcareous sponge Leucosolenia cf. variabilis. The regeneration of Leucosolenia cf. variabilis ends within 4–6 days. The crucial step of the process is the formation of the transient regenerative membrane, formed by the epithelial morphogenesis—spreading of the intact exopinacoderm and choanoderm. The spreading of the choanoderm is accompanied by the transdifferentiation of the choanocytes. The regenerative membrane develops without any contribution of the mesohyl cells. Subsequently, the membrane gradually transforms into the body wall. The cell proliferation is neither affected nor contributes to the regeneration at any stage. Thus, Leucosolenia cf. variabilis regeneration relies on the remodeling of the intact tissues through the epithelial morphogenesis, accompanied by the transdifferentiation of some differentiated cell types, which makes it similar to the regeneration in homoscleromorphs and eumetazoans.

KW - calcareous sponges, epithelial morphogenesis, regeneration, transdifferentiation

KW - calcareous sponges

KW - epithelial morphogenesis

KW - regeneration

KW - transdifferentiation

UR - http://www.scopus.com/inward/record.url?scp=85056328635&partnerID=8YFLogxK

UR - http://www.mendeley.com/research/sewing-up-wounds-epithelial-morphogenesis-central-mechanism-calcaronean-sponge-regeneration

U2 - 10.1002/jez.b.22830

DO - 10.1002/jez.b.22830

M3 - Article

VL - 330

SP - 351

EP - 371

JO - Journal of Experimental Zoology Part B: Molecular and Developmental Evolution

JF - Journal of Experimental Zoology Part B: Molecular and Developmental Evolution

SN - 1552-5007

IS - 6-7

ER -