TY - JOUR

T1 - Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region

AU - Lapidus, Alla

AU - Galleron, Nathalie

AU - Sorokin, Alexei

AU - Ehrlich, S. Dusko

PY - 1997

Y1 - 1997

N2 - The 200 kb region of the Bacillus subtilis chromosome spanning from 255 to 275°on the genetic map was sequenced. The strategy applied, based on use of yeast artificial chromosomes and multiplex Long Accurate PCR, proved to be very efficient for sequencing a large bacterial chromosome area. A total of 193 genes of this part of the chromosome was classified by level of knowledge and biological category of their functions. Five levels of gene function understanding are defined. These are: (i) experimental evidence is available of gene product or biological function; (ii) strong homology exists for the putative gene product with proteins from other organisms; (iii) some indication of the function can be derived from homologies with known proteins; (iv) the gene product can be clustered with hypothetical proteins; (v) no indication on the gene function exists. The percentage of detected genes in each category was: 20, 28, 20, 15 and 17, respectively. In the sequenced region, a high percentage of genes are implicated in transport and metabolic linking of glycolysis and the citric acid cycle. A functional connection of several genes from this region and the genes close to 140°in the chromosome was also observed.

AB - The 200 kb region of the Bacillus subtilis chromosome spanning from 255 to 275°on the genetic map was sequenced. The strategy applied, based on use of yeast artificial chromosomes and multiplex Long Accurate PCR, proved to be very efficient for sequencing a large bacterial chromosome area. A total of 193 genes of this part of the chromosome was classified by level of knowledge and biological category of their functions. Five levels of gene function understanding are defined. These are: (i) experimental evidence is available of gene product or biological function; (ii) strong homology exists for the putative gene product with proteins from other organisms; (iii) some indication of the function can be derived from homologies with known proteins; (iv) the gene product can be clustered with hypothetical proteins; (v) no indication on the gene function exists. The percentage of detected genes in each category was: 20, 28, 20, 15 and 17, respectively. In the sequenced region, a high percentage of genes are implicated in transport and metabolic linking of glycolysis and the citric acid cycle. A functional connection of several genes from this region and the genes close to 140°in the chromosome was also observed.

KW - Bacillus subtilis genome sequencing

KW - Long Accurate PCR

KW - Yeast artificial chromosome

UR - http://www.scopus.com/inward/record.url?scp=0030690274&partnerID=8YFLogxK

U2 - 10.1099/00221287-143-11-3431

DO - 10.1099/00221287-143-11-3431

M3 - Article

C2 - 9387221

AN - SCOPUS:0030690274

VL - 143

SP - 3431

EP - 3441

JO - Microbiology (United Kingdom)

JF - Microbiology (United Kingdom)

SN - 1350-0872

IS - 11

ER -