Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Self-assemble nanoparticles based on polypeptides containing C-terminal luminescent Pt-cysteine complex. / Vlakh, E. G.; Grachova, E. V.; Zhukovsky, D. D.; Hubina, A. V.; Mikhailova, A. S.; Shakirova, J. R.; Sharoyko, V. V.; Tunik, S. P.; Tennikova, T. B.
в: Scientific Reports, Том 7, 41991, 03.02.2017.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Self-assemble nanoparticles based on polypeptides containing C-terminal luminescent Pt-cysteine complex
AU - Vlakh, E. G.
AU - Grachova, E. V.
AU - Zhukovsky, D. D.
AU - Hubina, A. V.
AU - Mikhailova, A. S.
AU - Shakirova, J. R.
AU - Sharoyko, V. V.
AU - Tunik, S. P.
AU - Tennikova, T. B.
PY - 2017/2/3
Y1 - 2017/2/3
N2 - The growing attention to the luminescent nanocarriers is strongly stimulated by their potential application as drug delivery systems and by the necessity to monitor their distribution in cells and tissues. In this communication we report on the synthesis of amphiphilic polypeptides bearing C-terminal phosphorescent label together with preparation of nanoparticles using the polypeptides obtained. The approach suggested is based on a unique and highly technological process where the new phosphorescent Pt-cysteine complex serves as initiator of the ring-opening polymerization of a-amino acid N-carboxyanhydrides to obtain the polypeptides bearing intact the platinum chromophore covalently bound to the polymer chain. It was established that the luminescent label retains unchanged its emission characteristics not only in the polypeptides but also in more complicated nanoaggregates such as the polymer derived amphiphilic block-copolymers and self-assembled nanoparticles. The phosphorescent nanoparticles display no cytotoxicity and hemolytic activity in the tested range of concentrations and easily internalize into living cells that makes possible in vivo cell visualization, including prospective application in time resolved imaging and drug delivery monitoring.
AB - The growing attention to the luminescent nanocarriers is strongly stimulated by their potential application as drug delivery systems and by the necessity to monitor their distribution in cells and tissues. In this communication we report on the synthesis of amphiphilic polypeptides bearing C-terminal phosphorescent label together with preparation of nanoparticles using the polypeptides obtained. The approach suggested is based on a unique and highly technological process where the new phosphorescent Pt-cysteine complex serves as initiator of the ring-opening polymerization of a-amino acid N-carboxyanhydrides to obtain the polypeptides bearing intact the platinum chromophore covalently bound to the polymer chain. It was established that the luminescent label retains unchanged its emission characteristics not only in the polypeptides but also in more complicated nanoaggregates such as the polymer derived amphiphilic block-copolymers and self-assembled nanoparticles. The phosphorescent nanoparticles display no cytotoxicity and hemolytic activity in the tested range of concentrations and easily internalize into living cells that makes possible in vivo cell visualization, including prospective application in time resolved imaging and drug delivery monitoring.
KW - CYCLOMETALATED PLATINUM(II) COMPLEXES
KW - TARGETED DRUG-DELIVERY
KW - IN-VIVO
KW - POLYMERIC NANOPARTICLES
KW - CANCER-THERAPY
KW - FLUORESCENT
KW - LIGANDS
KW - NANOSTRUCTURES
KW - VISUALIZATION
KW - CARRIERS
U2 - 10.1038/srep41991
DO - 10.1038/srep41991
M3 - статья
VL - 7
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
M1 - 41991
ER -
ID: 7734623