Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Selenium speciation in paired serum and cerebrospinal fluid samples. / Solovyev, Nikolay; Berthele, Achim; Michalke, Bernhard.
в: Analytical and Bioanalytical Chemistry, Том 405, 2013, стр. 1875-1884.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Selenium speciation in paired serum and cerebrospinal fluid samples
AU - Solovyev, Nikolay
AU - Berthele, Achim
AU - Michalke, Bernhard
PY - 2013
Y1 - 2013
N2 - Se speciation was performed in 24 individual paired serum and cerebrospinal fluid (CSF) samples from neurologically healthy persons. Strong anion exchange (SAX) separation, coupled to inductively coupled plasma–dynamic reaction cell–mass spectrometry (ICP-DRC-MS),was employed. Species identification was done by standard matched retention time, standard addition and by size exclusion chromatography followed from SAX (2-D SECSAX-ICP-DRC-MS) and by SAX followed from CE-ICPDRC-MS (2-D SAX-CE-ICP-DRC-MS). Limit of detection (LoD, 3×standard deviation (SD) of noise) was in the range of 0.026–0.031 μg/L for all investigated species and thus was set uniformly to 0.032 μg/L. Quality control for total Se determination was performed by analysing control materials “human serum” and “urine”, where determined values met target values. Several Se species were found in both sample types having following median values (sequence: serum/ CSF, each in μg Se/L): total Se, 58.39/0.86; selenoprotein P (SePP), 5.19/0.47; Se-methioni
AB - Se speciation was performed in 24 individual paired serum and cerebrospinal fluid (CSF) samples from neurologically healthy persons. Strong anion exchange (SAX) separation, coupled to inductively coupled plasma–dynamic reaction cell–mass spectrometry (ICP-DRC-MS),was employed. Species identification was done by standard matched retention time, standard addition and by size exclusion chromatography followed from SAX (2-D SECSAX-ICP-DRC-MS) and by SAX followed from CE-ICPDRC-MS (2-D SAX-CE-ICP-DRC-MS). Limit of detection (LoD, 3×standard deviation (SD) of noise) was in the range of 0.026–0.031 μg/L for all investigated species and thus was set uniformly to 0.032 μg/L. Quality control for total Se determination was performed by analysing control materials “human serum” and “urine”, where determined values met target values. Several Se species were found in both sample types having following median values (sequence: serum/ CSF, each in μg Se/L): total Se, 58.39/0.86; selenoprotein P (SePP), 5.19/0.47; Se-methioni
KW - Selenium speciation
KW - Cerebrospinal fluid
KW - Serum
KW - Thioredoxin reductase
KW - Glutathione peroxidase
U2 - 10.1007/s00216-012-6294-y
DO - 10.1007/s00216-012-6294-y
M3 - Article
VL - 405
SP - 1875
EP - 1884
JO - Analytical and Bioanalytical Chemistry
JF - Analytical and Bioanalytical Chemistry
SN - 1618-2642
ER -
ID: 5627276