TY - JOUR

T1 - Rotifer nervous system visualized by FMRFamide and 5-HT immunocytochemistry and confocal laser scanning microscopy

AU - Kotikova, EA

AU - Raikova, O

AU - Reuter, M

AU - Gustafsson, MKS

PY - 2005/9/1

Y1 - 2005/9/1

N2 - We present the first results of immunocytochemical (ICC) observations on serotonin (5-HT) and FMRFamide (Phe-Met-Arg-Phe-NH2) immunoreactivity patterns in the rotifer nervous system investigated using a confocal laser scanning microscope (CLSM). Three species of rotifers are studied: Platyias patulus (Plationus patulits, Segers et al., 1993; Hydrobiologia 268: 1-8), Euchlanis dilatata, and Asplanchna herrickii. Independently from their systematic position, these species possess similar nerve structures. However, some differences were observed in the innervation of the corona, mastax, foot, and mostly in the pattern of the cerebral neurons. The general numbers of 5-HT-immunoreactive (IR) and FMRFamide-IR neurons are low (10-34), but constant for each species. The sizes of the neurons vary from 2, 5 to 10 mu m. From 4 to 14 cerebral neurons lie at different levels and are arranged into ail X- or a ring- or a curved arch shape. One or two pairs of neurons are localized along longitudinal nerve cords. Double staining of 5-HT and FMRFamide-IR elements shows no co-localization.

AB - We present the first results of immunocytochemical (ICC) observations on serotonin (5-HT) and FMRFamide (Phe-Met-Arg-Phe-NH2) immunoreactivity patterns in the rotifer nervous system investigated using a confocal laser scanning microscope (CLSM). Three species of rotifers are studied: Platyias patulus (Plationus patulits, Segers et al., 1993; Hydrobiologia 268: 1-8), Euchlanis dilatata, and Asplanchna herrickii. Independently from their systematic position, these species possess similar nerve structures. However, some differences were observed in the innervation of the corona, mastax, foot, and mostly in the pattern of the cerebral neurons. The general numbers of 5-HT-immunoreactive (IR) and FMRFamide-IR neurons are low (10-34), but constant for each species. The sizes of the neurons vary from 2, 5 to 10 mu m. From 4 to 14 cerebral neurons lie at different levels and are arranged into ail X- or a ring- or a curved arch shape. One or two pairs of neurons are localized along longitudinal nerve cords. Double staining of 5-HT and FMRFamide-IR elements shows no co-localization.

KW - Rotifera

KW - nervous system

KW - serotonin

KW - 5-HT

KW - FMRFamide

KW - immunocytochemistry

KW - CATECHOLAMINERGIC STRUCTURES

KW - MUSCULAR SYSTEMS

KW - LOCALIZATION

KW - ASCHELMINTHES

KW - NEUROPEPTIDE

KW - ACOELA

U2 - 10.1007/s10750-005-4203-5

DO - 10.1007/s10750-005-4203-5

M3 - статья

VL - 546

SP - 239

EP - 248

JO - Hydrobiologia

JF - Hydrobiologia

SN - 0018-8158

Y2 - 7 June 2003 through 13 June 2003

ER -