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Role of epsin 1 in synaptic vesicle endocytosis. / Jakobsson, Joel; Gad, Helge; Andersson, Fredrik; Löw, Peter; Shupliakov, Oleg; Brodin, Lennart.

в: Proceedings of the National Academy of Sciences of the United States of America, Том 105, № 17, 29.04.2008, стр. 6445-6450.

Результаты исследований: Научные публикации в периодических изданияхстатьяРецензирование

Harvard

Jakobsson, J, Gad, H, Andersson, F, Löw, P, Shupliakov, O & Brodin, L 2008, 'Role of epsin 1 in synaptic vesicle endocytosis', Proceedings of the National Academy of Sciences of the United States of America, Том. 105, № 17, стр. 6445-6450. https://doi.org/10.1073/pnas.0710267105

APA

Jakobsson, J., Gad, H., Andersson, F., Löw, P., Shupliakov, O., & Brodin, L. (2008). Role of epsin 1 in synaptic vesicle endocytosis. Proceedings of the National Academy of Sciences of the United States of America, 105(17), 6445-6450. https://doi.org/10.1073/pnas.0710267105

Vancouver

Jakobsson J, Gad H, Andersson F, Löw P, Shupliakov O, Brodin L. Role of epsin 1 in synaptic vesicle endocytosis. Proceedings of the National Academy of Sciences of the United States of America. 2008 Апр. 29;105(17):6445-6450. https://doi.org/10.1073/pnas.0710267105

Author

Jakobsson, Joel ; Gad, Helge ; Andersson, Fredrik ; Löw, Peter ; Shupliakov, Oleg ; Brodin, Lennart. / Role of epsin 1 in synaptic vesicle endocytosis. в: Proceedings of the National Academy of Sciences of the United States of America. 2008 ; Том 105, № 17. стр. 6445-6450.

BibTeX

@article{a11e77aa9b59431fb2b7f423572e7a38,
title = "Role of epsin 1 in synaptic vesicle endocytosis",
abstract = "Epsin has been suggested to act as an alternate adaptor in several endocytic pathways. Its role in synaptic vesicle recycling remains, however, unclear. Here, we examined the role of epsin in this process by using the lamprey reticulospinal synapse as a model system. We characterized a lamprey ortholog of epsin 1 and showed that it is accumulated at release sites at rest and also at clathrin-coated pits in the periactive zone during synaptic activity. Disruption of epsin interactions, by presynaptic microinjection of antibodies to either the epsin-N-terminal homology domain (ENTH) or the clathrin/AP2 binding region (CLAP), caused profound loss of vesicles in stimulated synapses. CLAP antibody-injected synapses displayed a massive accumulation of distorted coated structures, including coated vacuoles, whereas in synapses perturbed with ENTH antibodies, very few coated structures were found. In both cases coated pits on the plasma membrane showed a shift to early intermediates (shallow coated pits) and an increase in size. Moreover, in CLAP antibody-injected synapses flat clathrin-coated patches occurred on the plasma membrane. We conclude that epsin is involved in clathrin-mediated synaptic vesicle endocytosis. Our results support a model, based on in vitro studies, suggesting that epsin coordinates curvature generation with coat assembly and further indicating that epsin limits clathrin coat assembly to the size of newly formed vesicles. We propose that these functions of epsin 1 provide an additional mechanism for generation of uniformly sized synaptic vesicles.",
keywords = "Adaptor, Amphiphysin, AP2, Clathrin, ENTH domain",
author = "Joel Jakobsson and Helge Gad and Fredrik Andersson and Peter L{\"o}w and Oleg Shupliakov and Lennart Brodin",
year = "2008",
month = apr,
day = "29",
doi = "10.1073/pnas.0710267105",
language = "English",
volume = "105",
pages = "6445--6450",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "National Academy of Sciences",
number = "17",

}

RIS

TY - JOUR

T1 - Role of epsin 1 in synaptic vesicle endocytosis

AU - Jakobsson, Joel

AU - Gad, Helge

AU - Andersson, Fredrik

AU - Löw, Peter

AU - Shupliakov, Oleg

AU - Brodin, Lennart

PY - 2008/4/29

Y1 - 2008/4/29

N2 - Epsin has been suggested to act as an alternate adaptor in several endocytic pathways. Its role in synaptic vesicle recycling remains, however, unclear. Here, we examined the role of epsin in this process by using the lamprey reticulospinal synapse as a model system. We characterized a lamprey ortholog of epsin 1 and showed that it is accumulated at release sites at rest and also at clathrin-coated pits in the periactive zone during synaptic activity. Disruption of epsin interactions, by presynaptic microinjection of antibodies to either the epsin-N-terminal homology domain (ENTH) or the clathrin/AP2 binding region (CLAP), caused profound loss of vesicles in stimulated synapses. CLAP antibody-injected synapses displayed a massive accumulation of distorted coated structures, including coated vacuoles, whereas in synapses perturbed with ENTH antibodies, very few coated structures were found. In both cases coated pits on the plasma membrane showed a shift to early intermediates (shallow coated pits) and an increase in size. Moreover, in CLAP antibody-injected synapses flat clathrin-coated patches occurred on the plasma membrane. We conclude that epsin is involved in clathrin-mediated synaptic vesicle endocytosis. Our results support a model, based on in vitro studies, suggesting that epsin coordinates curvature generation with coat assembly and further indicating that epsin limits clathrin coat assembly to the size of newly formed vesicles. We propose that these functions of epsin 1 provide an additional mechanism for generation of uniformly sized synaptic vesicles.

AB - Epsin has been suggested to act as an alternate adaptor in several endocytic pathways. Its role in synaptic vesicle recycling remains, however, unclear. Here, we examined the role of epsin in this process by using the lamprey reticulospinal synapse as a model system. We characterized a lamprey ortholog of epsin 1 and showed that it is accumulated at release sites at rest and also at clathrin-coated pits in the periactive zone during synaptic activity. Disruption of epsin interactions, by presynaptic microinjection of antibodies to either the epsin-N-terminal homology domain (ENTH) or the clathrin/AP2 binding region (CLAP), caused profound loss of vesicles in stimulated synapses. CLAP antibody-injected synapses displayed a massive accumulation of distorted coated structures, including coated vacuoles, whereas in synapses perturbed with ENTH antibodies, very few coated structures were found. In both cases coated pits on the plasma membrane showed a shift to early intermediates (shallow coated pits) and an increase in size. Moreover, in CLAP antibody-injected synapses flat clathrin-coated patches occurred on the plasma membrane. We conclude that epsin is involved in clathrin-mediated synaptic vesicle endocytosis. Our results support a model, based on in vitro studies, suggesting that epsin coordinates curvature generation with coat assembly and further indicating that epsin limits clathrin coat assembly to the size of newly formed vesicles. We propose that these functions of epsin 1 provide an additional mechanism for generation of uniformly sized synaptic vesicles.

KW - Adaptor

KW - Amphiphysin

KW - AP2

KW - Clathrin

KW - ENTH domain

UR - http://www.scopus.com/inward/record.url?scp=44049105354&partnerID=8YFLogxK

U2 - 10.1073/pnas.0710267105

DO - 10.1073/pnas.0710267105

M3 - Article

C2 - 18430801

AN - SCOPUS:44049105354

VL - 105

SP - 6445

EP - 6450

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 17

ER -

ID: 40831437