Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Role of arginine deiminase in thymic atrophy during experimental Streptococcus pyogenes infection. / Starikova, Eleonora Alexandrovna; Golovin, Alexander Stanislavovich; Vasilyev, Kirill Alexandrovich; Karaseva, Alena Borisovna; Serebriakova, Maria Konstantinovna; Sokolov, Alexey Victorovich; Kudryavtsev, Igor Vladimirovich; Burova, Larissa Alexandrovna; Voynova, Irina Vitalyevna; Suvorov, Alexander Nikolaevich; Vasilyev, Vadim Borisovich; Freidlin, Irina Solomonovna.
в: Scandinavian Journal of Immunology, Том 89, № 2, e12734, 01.02.2019.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Role of arginine deiminase in thymic atrophy during experimental Streptococcus pyogenes infection
AU - Starikova, Eleonora Alexandrovna
AU - Golovin, Alexander Stanislavovich
AU - Vasilyev, Kirill Alexandrovich
AU - Karaseva, Alena Borisovna
AU - Serebriakova, Maria Konstantinovna
AU - Sokolov, Alexey Victorovich
AU - Kudryavtsev, Igor Vladimirovich
AU - Burova, Larissa Alexandrovna
AU - Voynova, Irina Vitalyevna
AU - Suvorov, Alexander Nikolaevich
AU - Vasilyev, Vadim Borisovich
AU - Freidlin, Irina Solomonovna
PY - 2019/2/1
Y1 - 2019/2/1
N2 - Expression of gene of arginine deiminase (AD) allows adaptation of Streptococcus pyogenes to adverse environmental conditions. AD activity can lead to L-arginine deficiency in the host cells’ microenvironment. Bioavailability of L-arginine is an important factor regulating the functions of the immune cells in mammals. By introducing a mutation into S pyogenes M46-16, we obtained a strain with inactivated arcA/sagp gene (M49-16 delArcA), deficient in AD. This allowed elucidating the function of AD in pathogenesis of streptococcal infection. The virulence of the parental and mutant strains was examined in a murine model of subcutaneous streptococcal infection. L-arginine concentration in the plasma of mice infected with S pyogenes M49-16 delArcA remained unchanged in course of the entire experiment. At the same time mice infected with S pyogenes M49-16 demonstrated gradual diminution of L-arginine concentration in the blood plasma, which might be due to the activity of streptococcal AD. Mice infected with S pyogenes M49-16 delArcA demonstrated less intensive bacterial growth in the primary foci and less pronounced bacterial dissemination as compared with animals infected with the parental strain S pyogenes M46-16. Similarly, thymus involution, alterations in apoptosis, thymocyte subsets and Treg cells differentiation were less pronounced in mice infected with S pyogenes M49-16 delArcA than in those infected with the parental strain. The results obtained showed that S pyogenes M49-16 delArcA, unable to produce AD, had reduced virulence in comparison with the parental S pyogenes M49-16 strain. AD is an important factor for the realization of the pathogenic potential of streptococci.
AB - Expression of gene of arginine deiminase (AD) allows adaptation of Streptococcus pyogenes to adverse environmental conditions. AD activity can lead to L-arginine deficiency in the host cells’ microenvironment. Bioavailability of L-arginine is an important factor regulating the functions of the immune cells in mammals. By introducing a mutation into S pyogenes M46-16, we obtained a strain with inactivated arcA/sagp gene (M49-16 delArcA), deficient in AD. This allowed elucidating the function of AD in pathogenesis of streptococcal infection. The virulence of the parental and mutant strains was examined in a murine model of subcutaneous streptococcal infection. L-arginine concentration in the plasma of mice infected with S pyogenes M49-16 delArcA remained unchanged in course of the entire experiment. At the same time mice infected with S pyogenes M49-16 demonstrated gradual diminution of L-arginine concentration in the blood plasma, which might be due to the activity of streptococcal AD. Mice infected with S pyogenes M49-16 delArcA demonstrated less intensive bacterial growth in the primary foci and less pronounced bacterial dissemination as compared with animals infected with the parental strain S pyogenes M46-16. Similarly, thymus involution, alterations in apoptosis, thymocyte subsets and Treg cells differentiation were less pronounced in mice infected with S pyogenes M49-16 delArcA than in those infected with the parental strain. The results obtained showed that S pyogenes M49-16 delArcA, unable to produce AD, had reduced virulence in comparison with the parental S pyogenes M49-16 strain. AD is an important factor for the realization of the pathogenic potential of streptococci.
KW - Animals
KW - Apoptosis
KW - Arginine/metabolism
KW - Atrophy
KW - Bacterial Proteins/genetics
KW - Cell Differentiation
KW - Cells, Cultured
KW - Disease Models, Animal
KW - Humans
KW - Hydrolases/genetics
KW - Mice
KW - Mice, Inbred C57BL
KW - Mice, Inbred CBA
KW - Mutagenesis, Site-Directed
KW - Mutation/genetics
KW - Streptococcal Infections/immunology
KW - Streptococcus pyogenes/pathogenicity
KW - T-Lymphocytes/physiology
KW - Thymus Gland/pathology
KW - Virulence
KW - SYSTEM
KW - APOPTOSIS
KW - INDUCTION
KW - VIRUS INFECTION
KW - REGULATORY T-CELLS
KW - MICE
KW - THYMOCYTES
KW - PROTEINS
KW - EXPRESSION
UR - http://www.scopus.com/inward/record.url?scp=85059593353&partnerID=8YFLogxK
U2 - 10.1111/sji.12734
DO - 10.1111/sji.12734
M3 - Article
C2 - 30471128
AN - SCOPUS:85059593353
VL - 89
JO - Scandinavian Journal of Immunology
JF - Scandinavian Journal of Immunology
SN - 0300-9475
IS - 2
M1 - e12734
ER -
ID: 42246630