TY - JOUR

T1 - Rhizosphere bacterium rhodococcus sp. P1y metabolizes ab-scisic acid to form dehydrovomifoliol

AU - Yuzikhin, Oleg S.

AU - Gogoleva, Natalia E.

AU - Shaposhnikov, Alexander I.

AU - Konnova, Tatyana A.

AU - Osipova, Elena V.

AU - Syrova, Darya S.

AU - Ermakova, Elena A.

AU - Shevchenko, Valerii P.

AU - Nagaev, Igor Yu

AU - Shevchenko, Konstantin V.

AU - Myasoedov, Nikolay F.

AU - Safronova, Vera I.

AU - Shavarda, Alexey L.

AU - Nizhnikov, Anton A.

AU - Belimov, Andrey A.

AU - Gogolev, Yuri V.

N1 - Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/3

Y1 - 2021/3

N2 - The phytohormone abscisic acid (ABA) plays an important role in plant growth and in response to abiotic stress factors. At the same time, its accumulation in soil can negatively affect seed germination, inhibit root growth and increase plant sensitivity to pathogens. ABA is an inert compound resistant to spontaneous hydrolysis and its biological transformation is scarcely under-stood. Recently, the strain Rhodococcus sp. P1Y was described as a rhizosphere bacterium assimilat-ing ABA as a sole carbon source in batch culture and affecting ABA concentrations in plant roots. In this work, the intermediate product of ABA decomposition by this bacterium was isolated and purified by preparative HPLC techniques. Proof that this compound belongs to ABA derivatives was carried out by measuring the molar radioactivity of the conversion products of this phytohor-mone labeled with tritium. The chemical structure of this compound was determined by instrumen-tal techniques including high-resolution mass spectrometry, NMR spectrometry, FTIR and UV spec-troscopies. As a result, the metabolite was identified as (4RS)-4-hydroxy-3,5,5-trimethyl-4-[(E)-3-oxobut-1-enyl]cyclohex-2-en-1-one (dehydrovomifoliol). Based on the data obtained, it was con-cluded that the pathway of bacterial degradation and assimilation of ABA begins with a gradual shortening of the acyl part of the molecule.

AB - The phytohormone abscisic acid (ABA) plays an important role in plant growth and in response to abiotic stress factors. At the same time, its accumulation in soil can negatively affect seed germination, inhibit root growth and increase plant sensitivity to pathogens. ABA is an inert compound resistant to spontaneous hydrolysis and its biological transformation is scarcely under-stood. Recently, the strain Rhodococcus sp. P1Y was described as a rhizosphere bacterium assimilat-ing ABA as a sole carbon source in batch culture and affecting ABA concentrations in plant roots. In this work, the intermediate product of ABA decomposition by this bacterium was isolated and purified by preparative HPLC techniques. Proof that this compound belongs to ABA derivatives was carried out by measuring the molar radioactivity of the conversion products of this phytohor-mone labeled with tritium. The chemical structure of this compound was determined by instrumen-tal techniques including high-resolution mass spectrometry, NMR spectrometry, FTIR and UV spec-troscopies. As a result, the metabolite was identified as (4RS)-4-hydroxy-3,5,5-trimethyl-4-[(E)-3-oxobut-1-enyl]cyclohex-2-en-1-one (dehydrovomifoliol). Based on the data obtained, it was con-cluded that the pathway of bacterial degradation and assimilation of ABA begins with a gradual shortening of the acyl part of the molecule.

KW - Abscisic acid

KW - Dehydrovomifoliol

KW - Microbial metabolite

KW - NMR spectrometry

KW - Phytohor-mones

KW - Rhizosphere

KW - Rhodococcus

UR - http://www.scopus.com/inward/record.url?scp=85101288513&partnerID=8YFLogxK

U2 - 10.3390/biom11030345

DO - 10.3390/biom11030345

M3 - Article

C2 - 33668728

AN - SCOPUS:85101288513

VL - 11

SP - 1

EP - 16

JO - Biomolecules

JF - Biomolecules

SN - 2218-273X

IS - 3

M1 - 345

ER -