TY - JOUR

T1 - Reproduction of trematodes in the molluscan host: an ultrastructural study of the germinal mass and brood cavity in daughter rediae of Tristriata anatis Belopolskaia, 1953 (Digenea: Notocotylidae)

AU - Подвязная, Ирина

AU - Галактионов, Кирилл Владимирович

N1 - Funding Information: Acknowledgements We are grateful to Natalia Lentsman for her help with the translation of the MS into English. We would like to thank the reviewers for their valuable comments and suggestions. Scientific research was performed using equipment of the BTaxon^ Research Resource Center (http://www.ckp-rf.ru/ckp/3038/) of the Zoological Institute of the Russian Academy of Sciences (St. Petersburg). Funding Information: Funding information This work was supported by the Russian Foundation for Basic Research (Grant No 16-04-00753) and by the programme of the Russian Academy of Sciences of the Zoological Institute No. АААА-А17-117030310322-3.

PY - 2018/8

Y1 - 2018/8

N2 - This study describes the fine structure of the germinal mass in daughter rediae of Tristriata anatis. The germinal mass consists of undifferentiated cells, germinal cells and supporting cells and contains numerous cercarial embryos up to tail bud stage. Supporting cells and their outgrowths form a tight meshwork of the germinal mass. In its basal part, this meshwork serves as scaffolding for undifferentiated and germinal cells, naked cell aggregates and early germinal balls. More mature embryos are located apically. The hypertrophied supporting tissue appears to be involved in an intensive transport of substances, as idicatedby abundant gap junctions between cell outgrowths and numerous pinocytotic vesicles and microtubules in their cytoplasm. Germinal cells contain annulate lamellae and the nuage, typical organelles of animal oocytes. In young rediae containing embryonic cercariae at the tail bud stage, the supporting tissue starts to degenerate in the apical part of the germinal mass, and a primordial brood cavity emerges though it develops fully only in mature rediae containing late embryonic cercariae. An unusual feature of the germinal mass in T. anatis rediae is an enhancement of the embryo brooding function. At the same time, theperformance of this function by the brood cavity is reduced. This is the first time such a redistribution of the embryo brooding function between the germinal mass and the brood cavity has been reported.

AB - This study describes the fine structure of the germinal mass in daughter rediae of Tristriata anatis. The germinal mass consists of undifferentiated cells, germinal cells and supporting cells and contains numerous cercarial embryos up to tail bud stage. Supporting cells and their outgrowths form a tight meshwork of the germinal mass. In its basal part, this meshwork serves as scaffolding for undifferentiated and germinal cells, naked cell aggregates and early germinal balls. More mature embryos are located apically. The hypertrophied supporting tissue appears to be involved in an intensive transport of substances, as idicatedby abundant gap junctions between cell outgrowths and numerous pinocytotic vesicles and microtubules in their cytoplasm. Germinal cells contain annulate lamellae and the nuage, typical organelles of animal oocytes. In young rediae containing embryonic cercariae at the tail bud stage, the supporting tissue starts to degenerate in the apical part of the germinal mass, and a primordial brood cavity emerges though it develops fully only in mature rediae containing late embryonic cercariae. An unusual feature of the germinal mass in T. anatis rediae is an enhancement of the embryo brooding function. At the same time, theperformance of this function by the brood cavity is reduced. This is the first time such a redistribution of the embryo brooding function between the germinal mass and the brood cavity has been reported.

KW - Tristriata anatis, reproduction of redia, ultrastructure, germinal mass, germinal cell, brood cavity

KW - Tristriata anatis . Reproduction of redia . Ultrastructure . Germinal mass . Germinal cell . Brood cavity

KW - Brood cavity

KW - Germinal mass

KW - Tristriata anatis

KW - Ultrastructure

KW - Germinal cell

KW - Reproduction of redia

KW - LIFE-CYCLE

KW - SYSTEM

KW - PRIMITIVE EPITHELIUM

KW - CERCARIA

KW - SPOROCYST

KW - ECHINOSTOMATIDAE

KW - ORIGIN

KW - EVOLUTION

KW - BUCEPHALIDAE

KW - OOCYTES

UR - http://www.scopus.com/inward/record.url?scp=85048270558&partnerID=8YFLogxK

UR - http://www.mendeley.com/research/reproduction-trematodes-molluscan-host-ultrastructural-study-germinal-mass-brood-cavity-daughter-red

U2 - 10.1007/s00436-018-5956-x

DO - 10.1007/s00436-018-5956-x

M3 - Article

VL - 117

SP - 2643

EP - 2652

JO - Parasitology Research

JF - Parasitology Research

SN - 0932-0113

IS - 8

ER -