TY - JOUR

T1 - Regulation of plasmodesmata in Arabidopsis leaves

T2 - ATP, NADPH and chlorophyll b levels matter

AU - Dmitrieva, Valeria A.

AU - Domashkina, Valentina V.

AU - Ivanova, Alexandra N.

AU - Sukhov, Vladimir S.

AU - Tyutereva, Elena V.

AU - Voitsekhovskaja, Olga V.

N1 - Publisher Copyright: © 2021 The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved.

PY - 2021/7/28

Y1 - 2021/7/28

N2 - In mature leaves, cell-to-cell transport via plasmodesmata between mesophyll cells links the production of assimilates by photosynthesis with their export to sink organs. This study addresses the question of how signals derived from chloroplasts and photosynthesis influence plasmodesmata permeability. Cell-to-cell transport was analyzed in leaves of the Arabidopsis chlorophyll b-less ch1-3 mutant, the same mutant complemented with a cyanobacterial CAO gene (PhCAO) overaccumulating chlorophyll b, the trxm3 mutant lacking plastidial thioredoxin m3, and the ntrc mutant lacking functional NADPH:thioredoxin reductase C. The regulation of plasmodesmata permeability in these lines could not be traced back to the reduction state of the thioredoxin system or the types and levels of reactive oxygen species produced in chloroplasts; however, it could be related to chloroplast ATP and NADPH production. The results suggest that light enables plasmodesmata closure via an increase in the ATP and NADPH levels produced in photosynthesis, providing a control mechanism for assimilate export based on the rate of photosynthate production in the Calvin-Benson cycle. The level of chlorophyll b influences plasmodesmata permeability via as-yet-unidentified signals. The data also suggest a role of thioredoxin m3 in the regulation of cyclic electron flow around photosystem I.

AB - In mature leaves, cell-to-cell transport via plasmodesmata between mesophyll cells links the production of assimilates by photosynthesis with their export to sink organs. This study addresses the question of how signals derived from chloroplasts and photosynthesis influence plasmodesmata permeability. Cell-to-cell transport was analyzed in leaves of the Arabidopsis chlorophyll b-less ch1-3 mutant, the same mutant complemented with a cyanobacterial CAO gene (PhCAO) overaccumulating chlorophyll b, the trxm3 mutant lacking plastidial thioredoxin m3, and the ntrc mutant lacking functional NADPH:thioredoxin reductase C. The regulation of plasmodesmata permeability in these lines could not be traced back to the reduction state of the thioredoxin system or the types and levels of reactive oxygen species produced in chloroplasts; however, it could be related to chloroplast ATP and NADPH production. The results suggest that light enables plasmodesmata closure via an increase in the ATP and NADPH levels produced in photosynthesis, providing a control mechanism for assimilate export based on the rate of photosynthate production in the Calvin-Benson cycle. The level of chlorophyll b influences plasmodesmata permeability via as-yet-unidentified signals. The data also suggest a role of thioredoxin m3 in the regulation of cyclic electron flow around photosystem I.

KW - Assimilate export

KW - ATP

KW - callose

KW - chlorophyll b

KW - cyclic electron flow

KW - far-red light

KW - NADPH

KW - photosystem I

KW - plasmodesmata

KW - reactive oxygen species

KW - thioredoxin m3

UR - http://www.scopus.com/inward/record.url?scp=85111501989&partnerID=8YFLogxK

U2 - 10.1093/jxb/erab205

DO - 10.1093/jxb/erab205

M3 - Article

AN - SCOPUS:85111501989

VL - 72

SP - 5534

EP - 5552

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

SN - 0022-0957

IS - 15

ER -