Red-shifted fluorescence from polyguanylic acid in aqueous solution at room temperature

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Red-shifted fluorescence from polyguanylic acid in aqueous solution at room temperature. / Kononov, A. I.; Bakulev, V. M.

в: Journal of Photochemistry and Photobiology B: Biology, Том 34, № 2-3, 01.01.1996, стр. 211-216.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

BibTeX

@article{d5ed0cf33a0a4fb6965cdc3252ebadf2,

title = "Red-shifted fluorescence from polyguanylic acid in aqueous solution at room temperature",

abstract = "The absorption, fluorescence emission, fluorescence excitation and fluorescence polarization spectra of polyriboguanylic acid (poly(G)) were studied in aqueous solution at room temperature. Greatly red-shifted fluorescence from poly(G) in water was attributed to a small amount of the bases protonated on the N(7) atom. This was supported by the following points: (1) the fluorescence emission spectrum of guanosine 5'-monophosphate in acid pH conditions was very close to that of poly(G) in water, and their fluorescence excitation spectra were coincident, and (2) the red-shifted fluorescence band disappeared on increasing pH with the addition of tris(hydroxymethyl) amino methane. The fluorescence titration curve exhibited a maximum at pH 4.8 that was assigned to the four-stranded form of poly(G), with part of the bases being protonated. The protonation degree was found to depend on the ionic strength of the solution.",

keywords = "Fluorescence, Fluorescence excitation spectra, Guanine cations, Polyguanylic acid, Protonation",

author = "Kononov, {A. I.} and Bakulev, {V. M.}",

year = "1996",

month = jan,

day = "1",

doi = "10.1016/1011-1344(96)07288-0",

language = "English",

volume = "34",

pages = "211--216",

journal = "Journal of Photochemistry and Photobiology B: Biology",

issn = "1011-1344",

publisher = "Elsevier",

number = "2-3",

}

RIS

TY - JOUR

T1 - Red-shifted fluorescence from polyguanylic acid in aqueous solution at room temperature

AU - Kononov, A. I.

AU - Bakulev, V. M.

PY - 1996/1/1

Y1 - 1996/1/1

N2 - The absorption, fluorescence emission, fluorescence excitation and fluorescence polarization spectra of polyriboguanylic acid (poly(G)) were studied in aqueous solution at room temperature. Greatly red-shifted fluorescence from poly(G) in water was attributed to a small amount of the bases protonated on the N(7) atom. This was supported by the following points: (1) the fluorescence emission spectrum of guanosine 5'-monophosphate in acid pH conditions was very close to that of poly(G) in water, and their fluorescence excitation spectra were coincident, and (2) the red-shifted fluorescence band disappeared on increasing pH with the addition of tris(hydroxymethyl) amino methane. The fluorescence titration curve exhibited a maximum at pH 4.8 that was assigned to the four-stranded form of poly(G), with part of the bases being protonated. The protonation degree was found to depend on the ionic strength of the solution.

AB - The absorption, fluorescence emission, fluorescence excitation and fluorescence polarization spectra of polyriboguanylic acid (poly(G)) were studied in aqueous solution at room temperature. Greatly red-shifted fluorescence from poly(G) in water was attributed to a small amount of the bases protonated on the N(7) atom. This was supported by the following points: (1) the fluorescence emission spectrum of guanosine 5'-monophosphate in acid pH conditions was very close to that of poly(G) in water, and their fluorescence excitation spectra were coincident, and (2) the red-shifted fluorescence band disappeared on increasing pH with the addition of tris(hydroxymethyl) amino methane. The fluorescence titration curve exhibited a maximum at pH 4.8 that was assigned to the four-stranded form of poly(G), with part of the bases being protonated. The protonation degree was found to depend on the ionic strength of the solution.

KW - Fluorescence

KW - Fluorescence excitation spectra

KW - Guanine cations

KW - Polyguanylic acid

KW - Protonation

UR - http://www.scopus.com/inward/record.url?scp=0030199469&partnerID=8YFLogxK

U2 - 10.1016/1011-1344(96)07288-0

DO - 10.1016/1011-1344(96)07288-0

M3 - Article

AN - SCOPUS:0030199469

VL - 34

SP - 211

EP - 216

JO - Journal of Photochemistry and Photobiology B: Biology

JF - Journal of Photochemistry and Photobiology B: Biology

SN - 1011-1344

IS - 2-3

ER -

ID: 39285701