DOI

  • D. A. Gorbenko
  • A. V. Belashov
  • T. N. Belyaeva
  • E. S. Kornilova
  • I. V. Semenova
  • O. S. Vasyutinskii

In this paper we perform an analysis of changes in cellular morphology in response to photodynamic treatment in vitro. Experiments were performed by means of digital holographic tomography. 3D spatial distributions of refractive index in cells were obtained and main morphological parameters of cells were determined: volume, average height, membrane area. The cellular response to treatment at different doses was studied and changes in morphology were correlated with major cell death pathways, apoptosis and necrosis. The necrosis pathway was studied in dynamics. The data obtained by holographic tomography were validated using a standard test for the integrity of cell membranes, conducted using a confocal fluorescent microscope.

Язык оригиналаанглийский
Номер статьи012015
ЖурналJournal of Physics: Conference Series
Том1236
Номер выпуска1
DOI
СостояниеОпубликовано - 16 июл 2019
Опубликовано для внешнего пользованияДа
СобытиеInternational Conference on Emerging Trends in Applied and Computational Physics 2019, ETACP 2019 - Saint Petersburg, Российская Федерация
Продолжительность: 21 мар 201922 мар 2019

    Предметные области Scopus

  • Физика и астрономия (все)

ID: 76657655