TY - JOUR

T1 - Pyrococcus CH1, an obligate piezophilic hyperthermophile

T2 - Extending the upper pressure-temperature limits for life

AU - Zeng, Xiang

AU - Birrien, Jean Louis

AU - Fouquet, Yves

AU - Cherkashov, Georgy

AU - Jebbar, Mohamed

AU - Querellou, Joël

AU - Oger, Philippe

AU - Cambon-Bonavita, Marie Anne

AU - Xiao, Xiang

AU - Prieur, Daniel

PY - 2009/1/1

Y1 - 2009/1/1

N2 - A novel hydrothermal site was discovered in March 2007, on the mid-Atlantic ridge during the cruise Serpentine. At a depth of 4100 m, the site Ashadze is the deepest vent field known so far. Smoker samples were collected with the ROV Victor 6000 and processed in the laboratory for the enrichment of anaerobic heterotrophic microorganisms under high-temperature and high-hydrostatic pressure conditions. Strain CH1 was successfully isolated and assigned to the genus Pyrococcus, within the Euryarchaeota lineage within the Archaea domain. This organism grows within a temperature range of 80 to 108 °C and a pressure range of 20 to 120 MPa, with optima for 98 °C and 52 MPa respectively. Pyrococcus CH1 represents the first obligate piezophilic hyperthermophilic microorganism known so far. Comparisons of growth yields obtained under high-temperature/high-pressure conditions for relative organisms isolated from various depths, showed clear relationships between depth at origin and responses to hydrostatic pressure.

AB - A novel hydrothermal site was discovered in March 2007, on the mid-Atlantic ridge during the cruise Serpentine. At a depth of 4100 m, the site Ashadze is the deepest vent field known so far. Smoker samples were collected with the ROV Victor 6000 and processed in the laboratory for the enrichment of anaerobic heterotrophic microorganisms under high-temperature and high-hydrostatic pressure conditions. Strain CH1 was successfully isolated and assigned to the genus Pyrococcus, within the Euryarchaeota lineage within the Archaea domain. This organism grows within a temperature range of 80 to 108 °C and a pressure range of 20 to 120 MPa, with optima for 98 °C and 52 MPa respectively. Pyrococcus CH1 represents the first obligate piezophilic hyperthermophilic microorganism known so far. Comparisons of growth yields obtained under high-temperature/high-pressure conditions for relative organisms isolated from various depths, showed clear relationships between depth at origin and responses to hydrostatic pressure.

KW - Archaea

KW - Deep-sea hydrothermal vent

KW - Extremophile

KW - High hydrostatic pressure

KW - Thermococcales

UR - http://www.scopus.com/inward/record.url?scp=67949103703&partnerID=8YFLogxK

U2 - 10.1038/ismej.2009.21

DO - 10.1038/ismej.2009.21

M3 - Article

C2 - 19295639

AN - SCOPUS:67949103703

VL - 3

SP - 873

EP - 876

JO - ISME Journal

JF - ISME Journal

SN - 1751-7362

IS - 7

ER -