TY - JOUR

T1 - Proteomic Profile of the Bacterium Sinorhizobium meliloti Depends on Its Life Form and Host Plant Species

AU - Antonets, K. S.

AU - Onishchuk, O. P.

AU - Kurchak, O. N.

AU - Volkov, K. V.

AU - Lykholay, A. N.

AU - Andreeva, E. A.

AU - Andronov, E. E.

AU - Pinaev, A. G.

AU - Provorov, N. A.

AU - Nizhnikov, A. A.

PY - 2018/9/1

Y1 - 2018/9/1

N2 - Abstract—The importance of root nodule bacteria in biotechnology is determined by their distinctive feature: symbiotic nitrogen fixation resulting in the production of organic nitrogen-containing compounds. While interacting with host legume plants, the cells of these bacteria undergo global changes at all levels of expression of genetic information leading to the formation in root nodules of so-called bacteroids functioning as nitrogen fixation factories. The molecular mechanisms underlying plant-microbial symbiosis are actively investigated, and one of the most interesting and poorly studied aspects of this problem is the species-specificity of interaction between root nodule bacteria and host plants. In this work we have performed the proteomic analysis of the Sinorhizobium meliloti bacteroids isolated from two legume species: alfalfa (Medicago sativa L.) and yellow sweet clover (Melilotus officinalis L.). It has been shown that the S. meliloti bacteroids produce a lot of proteins (many of them associated with symbiosis) in a host-specific manner, i.e., only in certain host plant species. It has been demonstrated for the first time that the levels of expression in bacteroids of the genes encoding the ExoZ and MscL proteins responsible for the synthesis of surface lipopolysaccharides and formation of a large conductance mechanosensitive channel, respectively, depend on a host plant species that confirms the results of proteomic analysis. Overall, our data show that the regulation of bacteroid development by the host plant has species-specific features.

AB - Abstract—The importance of root nodule bacteria in biotechnology is determined by their distinctive feature: symbiotic nitrogen fixation resulting in the production of organic nitrogen-containing compounds. While interacting with host legume plants, the cells of these bacteria undergo global changes at all levels of expression of genetic information leading to the formation in root nodules of so-called bacteroids functioning as nitrogen fixation factories. The molecular mechanisms underlying plant-microbial symbiosis are actively investigated, and one of the most interesting and poorly studied aspects of this problem is the species-specificity of interaction between root nodule bacteria and host plants. In this work we have performed the proteomic analysis of the Sinorhizobium meliloti bacteroids isolated from two legume species: alfalfa (Medicago sativa L.) and yellow sweet clover (Melilotus officinalis L.). It has been shown that the S. meliloti bacteroids produce a lot of proteins (many of them associated with symbiosis) in a host-specific manner, i.e., only in certain host plant species. It has been demonstrated for the first time that the levels of expression in bacteroids of the genes encoding the ExoZ and MscL proteins responsible for the synthesis of surface lipopolysaccharides and formation of a large conductance mechanosensitive channel, respectively, depend on a host plant species that confirms the results of proteomic analysis. Overall, our data show that the regulation of bacteroid development by the host plant has species-specific features.

KW - bacteroid

KW - chromatography

KW - ExoZ

KW - HPLC

KW - MALDI

KW - mass spectrometry

KW - Medicago

KW - Melilotus

KW - MscL

KW - root nodule bacteria

KW - Sinorhizobium meliloti

KW - symbiotic nitrogen fixation

KW - LEGUME

KW - PROTEIN

KW - SPECIFICITY

KW - GENE-EXPRESSION

KW - SYMBIOSIS

UR - http://www.scopus.com/inward/record.url?scp=85055257225&partnerID=8YFLogxK

UR - http://link.springer.com/10.1134/S0026893318050035

UR - http://www.mendeley.com/research/proteomic-profile-bacterium-sinorhizobium-meliloti-depends-life-form-host-plant-species

U2 - 10.1134/S0026893318050035

DO - 10.1134/S0026893318050035

M3 - Article

AN - SCOPUS:85055257225

VL - 52

SP - 779

EP - 785

JO - Molecular Biology

JF - Molecular Biology

SN - 0026-8933

IS - 5

ER -