TY - JOUR

T1 - Proteome Map of Pea (Pisum sativum L.) Embryos Containing Different Amounts of Residual Chlorophylls

AU - Mamontova, Tatiana

AU - Lukasheva, Elena

AU - Mavropolo-Stolyarenko, Gregory

AU - Proksch, Carsten

AU - Bilova, Tatiana

AU - Kim, Ahyoung

AU - Babakov, Vladimir

AU - Grishina, Tatiana

AU - Hoehenwarter, Wolfgang

AU - Medvedev, Sergei

AU - Smolikova, Galina

AU - Frolov, Andrej

N1 - Funding Information: Funding: This research was funded by the Russian Science Foundation (project number 16-16-00026). Funding Information: Acknowledgments: The authors are grateful to Veronika Chantseva, Olga Shiroglazova, and Svetlana Milrud (St. Petersburg State University) for technical assistance. The St. Petersburg St. University Research Resource Center for Molecular and Cell Technologies is acknowledged for technical support.

PY - 2018/12/15

Y1 - 2018/12/15

N2 - Due to low culturing costs and high seed protein contents, legumes represent the main global source of food protein. Pea (Pisum sativum L.) is one of the major legume crops, impacting both animal feed and human nutrition. Therefore, the quality of pea seeds needs to be ensured in the context of sustainable crop production and nutritional efficiency. Apparently, changes in seed protein patterns might directly affect both of these aspects. Thus, here, we address the pea seed proteome in detail and provide, to the best of our knowledge, the most comprehensive annotation of the functions and intracellular localization of pea seed proteins. To address possible intercultivar differences, we compared seed proteomes of yellow- and green-seeded pea cultivars in a comprehensive case study. The analysis revealed totally 1938 and 1989 nonredundant proteins, respectively. Only 35 and 44 proteins, respectively, could be additionally identified after protamine sulfate precipitation (PSP), potentially indicating the high efficiency of our experimental workflow. Totally 981 protein groups were assigned to 34 functional classes, which were to a large extent differentially represented in yellow and green seeds. Closer analysis of these differences by processing of the data in KEGG and String databases revealed their possible relation to a higher metabolic status and reduced longevity of green seeds.

AB - Due to low culturing costs and high seed protein contents, legumes represent the main global source of food protein. Pea (Pisum sativum L.) is one of the major legume crops, impacting both animal feed and human nutrition. Therefore, the quality of pea seeds needs to be ensured in the context of sustainable crop production and nutritional efficiency. Apparently, changes in seed protein patterns might directly affect both of these aspects. Thus, here, we address the pea seed proteome in detail and provide, to the best of our knowledge, the most comprehensive annotation of the functions and intracellular localization of pea seed proteins. To address possible intercultivar differences, we compared seed proteomes of yellow- and green-seeded pea cultivars in a comprehensive case study. The analysis revealed totally 1938 and 1989 nonredundant proteins, respectively. Only 35 and 44 proteins, respectively, could be additionally identified after protamine sulfate precipitation (PSP), potentially indicating the high efficiency of our experimental workflow. Totally 981 protein groups were assigned to 34 functional classes, which were to a large extent differentially represented in yellow and green seeds. Closer analysis of these differences by processing of the data in KEGG and String databases revealed their possible relation to a higher metabolic status and reduced longevity of green seeds.

KW - Chlorophylls

KW - LC-MS-based proteomics

KW - Pea (pisum sativum L.)

KW - Proteome functional annotation

KW - Proteome map

KW - Seed proteomics

KW - Seeds

KW - seeds

KW - pea

KW - lc-ms-based proteomics

KW - proteome functional

KW - proteome map

KW - chlorophylls

KW - seed proteomics

KW - pisum sativum l

UR - http://www.scopus.com/inward/record.url?scp=85058762852&partnerID=8YFLogxK

UR - http://www.mendeley.com/research/proteome-map-pea-pisum-sativum-l-embryos-containing-different-amounts-residual-chlorophylls

U2 - 10.3390/ijms19124066

DO - 10.3390/ijms19124066

M3 - Article

VL - 19

SP - 4066

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1422-0067

IS - 12

M1 - 4066

ER -