TY - JOUR

T1 - Protein biochips based on macroporous polymer supports

T2 - Material properties and analytical potential

AU - Volokitina, Mariia

AU - Krutyakova, Mariia

AU - Sirotov, Vasilii

AU - Larionov, Maksim

AU - Tennikova, Tatiana

AU - Korzhikova-Vlakh, Evgenia

PY - 2019/2/20

Y1 - 2019/2/20

N2 - A series of rigid macroporous polymer layers differed by hydrophobic–hydrophilic properties was synthesized in situ in preliminary fabricated wells and applied as the platforms for protein biochips. Scanning electron microscopy, etalon porosimetry and BET analysis were used for materials characterization. The comparison of analytical efficiency of the developed platforms allowed for the choice of the most optimal polymer, as well as the evaluation of impact of material porous properties. The quantitative parameters of affinity interaction between two different protein pairs were calculated depending on biochip characteristics using the developed analytical protocol. Moreover, the described biochips were successfully tested to detect acetylcholinesterase via catalytic reaction followed by the formation of fluoresceine as a product. Different parameters of enzymatic reaction were calculated for the reaction on a chip and compared to those established for in solution process.

AB - A series of rigid macroporous polymer layers differed by hydrophobic–hydrophilic properties was synthesized in situ in preliminary fabricated wells and applied as the platforms for protein biochips. Scanning electron microscopy, etalon porosimetry and BET analysis were used for materials characterization. The comparison of analytical efficiency of the developed platforms allowed for the choice of the most optimal polymer, as well as the evaluation of impact of material porous properties. The quantitative parameters of affinity interaction between two different protein pairs were calculated depending on biochip characteristics using the developed analytical protocol. Moreover, the described biochips were successfully tested to detect acetylcholinesterase via catalytic reaction followed by the formation of fluoresceine as a product. Different parameters of enzymatic reaction were calculated for the reaction on a chip and compared to those established for in solution process.

KW - Acetylcholinesterase (AChE)

KW - Affinity binding

KW - Antigen-antibody interaction

KW - Biocatalytic reaction on a chip

KW - Biochips

KW - Dissociation constants

KW - Polymer macroporous monoliths

KW - Protein analysis

KW - Microscopy, Electron, Scanning

KW - Proteins/analysis

KW - Polymers/chemistry

KW - Protein Array Analysis/methods

KW - Acetylcholinesterase/analysis

KW - Hydrophobic and Hydrophilic Interactions

KW - Porosity

KW - MICROARRAY

KW - CHAIN-REACTION

KW - MONOLITHS

KW - STATIONARY PHASES

KW - SERUM

UR - http://www.scopus.com/inward/record.url?scp=85058392090&partnerID=8YFLogxK

U2 - 10.1016/j.jpba.2018.12.012

DO - 10.1016/j.jpba.2018.12.012

M3 - Article

C2 - 30557782

AN - SCOPUS:85058392090

VL - 165

SP - 242

EP - 250

JO - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

SN - 0731-7085

ER -