It is known that any surfaces in environment of healthcare organizations are a reservoir for microorganisms and contribute to transmission of pathogens, increasing the risk of cross-contamination through indirect contact with the patient. Wide and not always rational use of chemical disinfectants carries risks for the safety of the environment, the patient and medical staff. In this regard, there remains the search for alternative methods of purification and decontamination of abiotic surfaces in health care facilities. Tested the tool for cleaning areas that contain 3 strains of the genus Bacillus: B. subtillis, B. licheniformis, B. pumilus. Were treated areas of the medical centre in St. Petersburg Pasteur Institute: the treatment room (experience) and the office of the gynecologist (control with conventional cleaning and disinfection). Within 30 days of the sowing of samples of swabs from surfaces were made of 3 Petri dishes with the "blood" agar-based environment "agar of Givental-Witch" debilitating touch (gold method). Isolated cultures of bacteria identified by microscopy, biochemical typing and MALDI-TOF MS (Bruker). In control and the experience in the 1st day of observation prior to the cleaning sown Enterococcus faecium 10 ⁴ -10 ⁵ CFU/ml. The 2 ^nd day of observation in the control was dominated by E. faecium, then his growth stopped. For the 2 ^nd -4 ^th day watched the growth of Enterobacteriaceae to 10 ⁶ CFU/ml, in the future, the number was 10 CFU/ml until the end of the observation period (30 days). The 5 ^th day in the control revealed the growth of bacteria of the genus Staphylococcus spp. mainly S. epidermidis. The experience from the 2 ^nd day of observation revealed a significant increase in Bacillus spp. at 10 ⁵ CFU/ml, then before the end of the experiment, the number was 10 ² -10 ³ CFU/ml. In the 1 ^st -2 ^nd day revealed growth of E. faecium, and then it stopped. For the 2 ^nd -4 ^th day watched the growth of Enterobacteriaceae to the level of 10 ⁶ CFU/ml, on the 8 ^th day-to 10 ² CFU/ml, the growth of conditionally pathogenic microflora was absent. Cleaning of the premises on the basis of probiotic bacteria of the genus Bacillus inhibits the growth of sanitary-indicative microorganisms compared to control. Thus, the means of probiotic treatment did not have a biocidal effect, but inhabiting the surface, they inhibit the growth and proliferation of opportunistic bacteria.