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Preparation of lampbrush chromosomes dissected from avian and reptilian growing oocytes. / Saifitdinova, Alsu; Galkina, Svetlana; Volodkina, Valeriia; Gaginskaya, Elena.

в: Biological Communications, Том 62, № 3, 29.12.2017, стр. 165-168.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

Harvard

Saifitdinova, A, Galkina, S, Volodkina, V & Gaginskaya, E 2017, 'Preparation of lampbrush chromosomes dissected from avian and reptilian growing oocytes', Biological Communications, Том. 62, № 3, стр. 165-168. https://doi.org/10.21638/11701/spbu03.2017.302

APA

Saifitdinova, A., Galkina, S., Volodkina, V., & Gaginskaya, E. (2017). Preparation of lampbrush chromosomes dissected from avian and reptilian growing oocytes. Biological Communications, 62(3), 165-168. https://doi.org/10.21638/11701/spbu03.2017.302

Vancouver

Saifitdinova A, Galkina S, Volodkina V, Gaginskaya E. Preparation of lampbrush chromosomes dissected from avian and reptilian growing oocytes. Biological Communications. 2017 Дек. 29;62(3):165-168. https://doi.org/10.21638/11701/spbu03.2017.302

Author

Saifitdinova, Alsu ; Galkina, Svetlana ; Volodkina, Valeriia ; Gaginskaya, Elena. / Preparation of lampbrush chromosomes dissected from avian and reptilian growing oocytes. в: Biological Communications. 2017 ; Том 62, № 3. стр. 165-168.

BibTeX

@article{c331d0df22584b38989d07d7d44a80fb,
title = "Preparation of lampbrush chromosomes dissected from avian and reptilian growing oocytes",
abstract = "Lampbrush chromosomes represent transcriptionally active chromosomes that exist during the extended diplotene phase in the growing ovarian eggs of many vertebrate animals, except therian mammals. They are excellent for studying chromosome structure and aspects of RNA transcription. Because of their giant size they allow a great increase in the resolution for physical gene mapping. Lampbrush chromosomes have no connection with the nuclear envelope, and can be manually dissected from living oocyte nuclei. Here we present the complete protocol for lampbrush chromosome microsurgical isolation from avian and reptilian germinal vesicles. These chromosome preparations can be further used for fluorescence in situ hybridization (FISH) and immunostaining.",
keywords = "Aves, Germinal vesicle, Lampbrush chromosomes, Meiotic chromosomes, Microsurgical isolation, Nucleus, Reptilia",
author = "Alsu Saifitdinova and Svetlana Galkina and Valeriia Volodkina and Elena Gaginskaya",
year = "2017",
month = dec,
day = "29",
doi = "10.21638/11701/spbu03.2017.302",
language = "English",
volume = "62",
pages = "165--168",
journal = "Biological Communications",
issn = "2542-2154",
publisher = "Издательство Санкт-Петербургского университета",
number = "3",

}

RIS

TY - JOUR

T1 - Preparation of lampbrush chromosomes dissected from avian and reptilian growing oocytes

AU - Saifitdinova, Alsu

AU - Galkina, Svetlana

AU - Volodkina, Valeriia

AU - Gaginskaya, Elena

PY - 2017/12/29

Y1 - 2017/12/29

N2 - Lampbrush chromosomes represent transcriptionally active chromosomes that exist during the extended diplotene phase in the growing ovarian eggs of many vertebrate animals, except therian mammals. They are excellent for studying chromosome structure and aspects of RNA transcription. Because of their giant size they allow a great increase in the resolution for physical gene mapping. Lampbrush chromosomes have no connection with the nuclear envelope, and can be manually dissected from living oocyte nuclei. Here we present the complete protocol for lampbrush chromosome microsurgical isolation from avian and reptilian germinal vesicles. These chromosome preparations can be further used for fluorescence in situ hybridization (FISH) and immunostaining.

AB - Lampbrush chromosomes represent transcriptionally active chromosomes that exist during the extended diplotene phase in the growing ovarian eggs of many vertebrate animals, except therian mammals. They are excellent for studying chromosome structure and aspects of RNA transcription. Because of their giant size they allow a great increase in the resolution for physical gene mapping. Lampbrush chromosomes have no connection with the nuclear envelope, and can be manually dissected from living oocyte nuclei. Here we present the complete protocol for lampbrush chromosome microsurgical isolation from avian and reptilian germinal vesicles. These chromosome preparations can be further used for fluorescence in situ hybridization (FISH) and immunostaining.

KW - Aves

KW - Germinal vesicle

KW - Lampbrush chromosomes

KW - Meiotic chromosomes

KW - Microsurgical isolation

KW - Nucleus

KW - Reptilia

UR - http://www.scopus.com/inward/record.url?scp=85063302778&partnerID=8YFLogxK

U2 - 10.21638/11701/spbu03.2017.302

DO - 10.21638/11701/spbu03.2017.302

M3 - Article

AN - SCOPUS:85063302778

VL - 62

SP - 165

EP - 168

JO - Biological Communications

JF - Biological Communications

SN - 2542-2154

IS - 3

ER -

ID: 41713129