Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Preparation, characterization, and biological evaluation of poly(glutamic acid)-b-polyphenylalanine polymersomes. / Vlakh, Evgenia; Ananyan, Anastasiia; Zashikhina, Natalia; Hubina, Anastasiia; Pogodaev, Aleksander; Volokitina, Mariia; Sharoyko, Vladimir; Tennikova, Tatiana.
в: Polymers, Том 8, № 6, 212, 02.06.2016.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
}
TY - JOUR
T1 - Preparation, characterization, and biological evaluation of poly(glutamic acid)-b-polyphenylalanine polymersomes
AU - Vlakh, Evgenia
AU - Ananyan, Anastasiia
AU - Zashikhina, Natalia
AU - Hubina, Anastasiia
AU - Pogodaev, Aleksander
AU - Volokitina, Mariia
AU - Sharoyko, Vladimir
AU - Tennikova, Tatiana
N1 - Publisher Copyright: © 2016 by the author.
PY - 2016/6/2
Y1 - 2016/6/2
N2 - Different types of amphiphilic macromolecular structures have been developed within recent decades to prepare the polymer particles considered as drug delivery systems. In the present research the series of amphiphilic block-copolymers containing poly(glutamatic acid) as hydrophilic, and polyphenylalanine as hydrophobic blocks was synthesized and characterized. Molecular weights for homo- and copolymers were determined by gel-permeation chromatography (GPC) and amino acid analysis, respectively. The copolymers obtained were applied for preparation of polymer particles. The specific morphology of prepared polymerosomes was proved using transmission electron microscopy (TEM). The influence on particle size of polymer concentration and pH used for self-assembly, as well as on the length of hydrophobic and hydrophilic blocks of applied copolymers, was studied by dynamic light scattering (DLS). Depending on different experimental conditions, the formation of nanoparticles with sizes from 60 to 350 nm was observed. The surface of polymersomes was modified with model protein (enzyme). No loss in biocatalytic activity was detected. Additionally, the process of encapsulation of model dyes was developed and the possibility of intracellular delivery of the dye-loaded nanoparticles was proved. Thus, the nanoparticles discussed can be considered for the creation of modern drug delivery systems.
AB - Different types of amphiphilic macromolecular structures have been developed within recent decades to prepare the polymer particles considered as drug delivery systems. In the present research the series of amphiphilic block-copolymers containing poly(glutamatic acid) as hydrophilic, and polyphenylalanine as hydrophobic blocks was synthesized and characterized. Molecular weights for homo- and copolymers were determined by gel-permeation chromatography (GPC) and amino acid analysis, respectively. The copolymers obtained were applied for preparation of polymer particles. The specific morphology of prepared polymerosomes was proved using transmission electron microscopy (TEM). The influence on particle size of polymer concentration and pH used for self-assembly, as well as on the length of hydrophobic and hydrophilic blocks of applied copolymers, was studied by dynamic light scattering (DLS). Depending on different experimental conditions, the formation of nanoparticles with sizes from 60 to 350 nm was observed. The surface of polymersomes was modified with model protein (enzyme). No loss in biocatalytic activity was detected. Additionally, the process of encapsulation of model dyes was developed and the possibility of intracellular delivery of the dye-loaded nanoparticles was proved. Thus, the nanoparticles discussed can be considered for the creation of modern drug delivery systems.
KW - Amphiphilic copolymers
KW - Biodegradation
KW - Cell uptake
KW - Encapsulation
KW - Poly(amino acid)s
KW - Polymer particles
KW - Polymersomes
UR - http://www.scopus.com/inward/record.url?scp=84976500260&partnerID=8YFLogxK
U2 - 10.3390/polym8060212
DO - 10.3390/polym8060212
M3 - Article
VL - 8
JO - Polymers
JF - Polymers
SN - 2073-4360
IS - 6
M1 - 212
ER -
ID: 7569955