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Possibilities of X-Ray Absorption Spectroscopy in the Total External Reflection Geometry for Studying Protein Films on Liquids. / Novikova, N. N.; Yakunin, S. N.; Koval’chuk, M. V.; Yur’eva, E. A.; Stepina, N. D.; Rogachev, A. V.; Kremennaya, M. A.; Yalovega, G. E.; Kosmachevskaya, O. V.; Topunov, A. F.

в: Crystallography Reports, Том 64, № 6, 01.11.2019, стр. 952-957.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

Harvard

Novikova, NN, Yakunin, SN, Koval’chuk, MV, Yur’eva, EA, Stepina, ND, Rogachev, AV, Kremennaya, MA, Yalovega, GE, Kosmachevskaya, OV & Topunov, AF 2019, 'Possibilities of X-Ray Absorption Spectroscopy in the Total External Reflection Geometry for Studying Protein Films on Liquids', Crystallography Reports, Том. 64, № 6, стр. 952-957. https://doi.org/10.1134/S1063774519060130

APA

Novikova, N. N., Yakunin, S. N., Koval’chuk, M. V., Yur’eva, E. A., Stepina, N. D., Rogachev, A. V., Kremennaya, M. A., Yalovega, G. E., Kosmachevskaya, O. V., & Topunov, A. F. (2019). Possibilities of X-Ray Absorption Spectroscopy in the Total External Reflection Geometry for Studying Protein Films on Liquids. Crystallography Reports, 64(6), 952-957. https://doi.org/10.1134/S1063774519060130

Vancouver

Novikova NN, Yakunin SN, Koval’chuk MV, Yur’eva EA, Stepina ND, Rogachev AV и пр. Possibilities of X-Ray Absorption Spectroscopy in the Total External Reflection Geometry for Studying Protein Films on Liquids. Crystallography Reports. 2019 Нояб. 1;64(6):952-957. https://doi.org/10.1134/S1063774519060130

Author

Novikova, N. N. ; Yakunin, S. N. ; Koval’chuk, M. V. ; Yur’eva, E. A. ; Stepina, N. D. ; Rogachev, A. V. ; Kremennaya, M. A. ; Yalovega, G. E. ; Kosmachevskaya, O. V. ; Topunov, A. F. / Possibilities of X-Ray Absorption Spectroscopy in the Total External Reflection Geometry for Studying Protein Films on Liquids. в: Crystallography Reports. 2019 ; Том 64, № 6. стр. 952-957.

BibTeX

@article{b716499e15be43238670da2882b3d119,
title = "Possibilities of X-Ray Absorption Spectroscopy in the Total External Reflection Geometry for Studying Protein Films on Liquids",
abstract = "Abstract: XANES spectra of protein films (hemoglobin and alkaline phosphatase) formed on the surface of a liquid subphase in a Langmuir trough have been obtained experimentally. The potential of X-ray absorption spectroscopy in the total external reflection geometry is demonstrated by the example of a protein film based on hemoglobin subjected to the action of urea. It is established that the presence of 0.09 M urea solution in the subphase enhances significantly the ability of hemoglobin to bind zinc and iron ions. Information about the local atomic environment of zinc ions bound with a hemoglobin molecule is obtained. It is shown that each zinc ion is coordinated by four ligands, two of which are amino acid residues of cysteine and histidine. The general concepts of the molecular mechanisms of accumulation of metal ions under the action of disturbing factors are formulated.",
author = "Novikova, {N. N.} and Yakunin, {S. N.} and Koval{\textquoteright}chuk, {M. V.} and Yur{\textquoteright}eva, {E. A.} and Stepina, {N. D.} and Rogachev, {A. V.} and Kremennaya, {M. A.} and Yalovega, {G. E.} and Kosmachevskaya, {O. V.} and Topunov, {A. F.}",
note = "Publisher Copyright: {\textcopyright} 2019, Pleiades Publishing, Inc.",
year = "2019",
month = nov,
day = "1",
doi = "10.1134/S1063774519060130",
language = "English",
volume = "64",
pages = "952--957",
journal = "Crystallography Reports",
issn = "1063-7745",
publisher = "МАИК {"}Наука/Интерпериодика{"}",
number = "6",

}

RIS

TY - JOUR

T1 - Possibilities of X-Ray Absorption Spectroscopy in the Total External Reflection Geometry for Studying Protein Films on Liquids

AU - Novikova, N. N.

AU - Yakunin, S. N.

AU - Koval’chuk, M. V.

AU - Yur’eva, E. A.

AU - Stepina, N. D.

AU - Rogachev, A. V.

AU - Kremennaya, M. A.

AU - Yalovega, G. E.

AU - Kosmachevskaya, O. V.

AU - Topunov, A. F.

N1 - Publisher Copyright: © 2019, Pleiades Publishing, Inc.

PY - 2019/11/1

Y1 - 2019/11/1

N2 - Abstract: XANES spectra of protein films (hemoglobin and alkaline phosphatase) formed on the surface of a liquid subphase in a Langmuir trough have been obtained experimentally. The potential of X-ray absorption spectroscopy in the total external reflection geometry is demonstrated by the example of a protein film based on hemoglobin subjected to the action of urea. It is established that the presence of 0.09 M urea solution in the subphase enhances significantly the ability of hemoglobin to bind zinc and iron ions. Information about the local atomic environment of zinc ions bound with a hemoglobin molecule is obtained. It is shown that each zinc ion is coordinated by four ligands, two of which are amino acid residues of cysteine and histidine. The general concepts of the molecular mechanisms of accumulation of metal ions under the action of disturbing factors are formulated.

AB - Abstract: XANES spectra of protein films (hemoglobin and alkaline phosphatase) formed on the surface of a liquid subphase in a Langmuir trough have been obtained experimentally. The potential of X-ray absorption spectroscopy in the total external reflection geometry is demonstrated by the example of a protein film based on hemoglobin subjected to the action of urea. It is established that the presence of 0.09 M urea solution in the subphase enhances significantly the ability of hemoglobin to bind zinc and iron ions. Information about the local atomic environment of zinc ions bound with a hemoglobin molecule is obtained. It is shown that each zinc ion is coordinated by four ligands, two of which are amino acid residues of cysteine and histidine. The general concepts of the molecular mechanisms of accumulation of metal ions under the action of disturbing factors are formulated.

UR - http://www.scopus.com/inward/record.url?scp=85077028162&partnerID=8YFLogxK

U2 - 10.1134/S1063774519060130

DO - 10.1134/S1063774519060130

M3 - Article

AN - SCOPUS:85077028162

VL - 64

SP - 952

EP - 957

JO - Crystallography Reports

JF - Crystallography Reports

SN - 1063-7745

IS - 6

ER -

ID: 88198382