TY - JOUR

T1 - Polymorphism in Sequences of Agrobacterial Origin in Nicotiana tabacum Cultivars

AU - Khafizova, G. V.

AU - Matveeva, T. V.

N1 - Funding Information: This work was supported by a grant from the Russian Foundation for Basic Research (project no. 18-016-00118) using the equipment of the Centre for Molecular and Cell Technologies of the Research Park of St. Petersburg State University. Publisher Copyright: © 2020, Pleiades Publishing, Inc. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/10/1

Y1 - 2020/10/1

N2 - Nicotiana tabacumis a plant containing cellular T-DNA (T-DNA obtained by plants fromAgrobacteriumduring evolution as a result of horizontal gene transfer) in the genome. Within the speciesN. tabacum, many varieties are distinguished, and phylogenetic relationships among them have not been established yet. Despite the diversity of phenotypes,N. tabacumvarieties are known to have a low level of genetic diversity, so clarification of intraspecific phylogeny is complicated. This article proposes a new marker for studying the phylogeny of theNicotiana tabacumspecies on the basis of the results of a comparative analysis of the cT-DNA TA and TB fragments and thePMT2gene in 11 varieties ofNicotiana tabacum. It has been shown that, for sequences of agrobacterial origin in the genomes of plants, the same evolutionary mechanisms are typical, as for plant sequences. Thus, the cT-DNA (namely, TA) sequences can be used as a phylogenetic marker for the speciesN. tabacum, since the varieties can be separated into two groups based on the structure of the TA central region.

AB - Nicotiana tabacumis a plant containing cellular T-DNA (T-DNA obtained by plants fromAgrobacteriumduring evolution as a result of horizontal gene transfer) in the genome. Within the speciesN. tabacum, many varieties are distinguished, and phylogenetic relationships among them have not been established yet. Despite the diversity of phenotypes,N. tabacumvarieties are known to have a low level of genetic diversity, so clarification of intraspecific phylogeny is complicated. This article proposes a new marker for studying the phylogeny of theNicotiana tabacumspecies on the basis of the results of a comparative analysis of the cT-DNA TA and TB fragments and thePMT2gene in 11 varieties ofNicotiana tabacum. It has been shown that, for sequences of agrobacterial origin in the genomes of plants, the same evolutionary mechanisms are typical, as for plant sequences. Thus, the cT-DNA (namely, TA) sequences can be used as a phylogenetic marker for the speciesN. tabacum, since the varieties can be separated into two groups based on the structure of the TA central region.

KW - cT-DNA (cellular T-DNA)

KW - horizontal gene transfer

KW - Nicotiana tabacum

KW - EVOLUTION

KW - T-DNA

UR - http://www.scopus.com/inward/record.url?scp=85092394964&partnerID=8YFLogxK

U2 - 10.1134/S1022795420100051

DO - 10.1134/S1022795420100051

M3 - Article

AN - SCOPUS:85092394964

VL - 56

SP - 1269

EP - 1271

JO - Russian Journal of Genetics

JF - Russian Journal of Genetics

SN - 1022-7954

IS - 10

ER -