TY - JOUR

T1 - Plant heterotrophic cultures: No food, no growth

AU - Пузанский, Роман Константинович

AU - Романюк, Дарья Андреевна

AU - Кирпичникова, Анастасия Алексеевна

AU - Емельянов, Владислав Владимирович

AU - Шишова, Мария Федоровна

N1 - Puzanskiy R.K., Romanyuk D.A., Kirpichnikova A.A., Yemelyanov V.V., Shishova M.F. Plant heterotrophic cultures: No food, no growth // Plants. 2024. Vol. 13, No. 2. 277. https://doi.org/10.3390/plants13020277

PY - 2024/1/17

Y1 - 2024/1/17

N2 - Plant cells are capable of uptaking exogenous organic substances. This inherited trait allows the development of heterotrophic cell cultures in various plants. The most common of them are Nicotiana tabacum and Arabidopsis thaliana. Plant cells are widely used in academic studies and as factories for valuable substance production. The repertoire of compounds supporting the hetero-trophic growth of plant cells is limited. The best growth of cultures is ensured by oligosaccharides and their cleavage products. Primarily, these are sucrose, raffinose, glucose and fructose. Other molecules such as glycerol, carbonic acids, starch, and mannitol have the ability to support growth occasionally, or in combination with another substrate. Culture growth is accompanied by pro-cesses of specialization, such as elongation growth. This determines the pattern of the carbon budget. Culture ageing is closely linked to substrate depletion, changes in medium composition, and cell physiological rearrangements. A lack of substrate leads to starvation, which results in a decrease in physiological activity and the mobilization of resources, and finally in the loss of via-bility. The cause of the instability of cultivated cells may be the non-optimal metabolism under cultural conditions or the insufficiency of internal regulation.

AB - Plant cells are capable of uptaking exogenous organic substances. This inherited trait allows the development of heterotrophic cell cultures in various plants. The most common of them are Nicotiana tabacum and Arabidopsis thaliana. Plant cells are widely used in academic studies and as factories for valuable substance production. The repertoire of compounds supporting the hetero-trophic growth of plant cells is limited. The best growth of cultures is ensured by oligosaccharides and their cleavage products. Primarily, these are sucrose, raffinose, glucose and fructose. Other molecules such as glycerol, carbonic acids, starch, and mannitol have the ability to support growth occasionally, or in combination with another substrate. Culture growth is accompanied by pro-cesses of specialization, such as elongation growth. This determines the pattern of the carbon budget. Culture ageing is closely linked to substrate depletion, changes in medium composition, and cell physiological rearrangements. A lack of substrate leads to starvation, which results in a decrease in physiological activity and the mobilization of resources, and finally in the loss of via-bility. The cause of the instability of cultivated cells may be the non-optimal metabolism under cultural conditions or the insufficiency of internal regulation.

KW - plant heterotrophic culture

KW - sucrose

KW - organic uptake

KW - expansion growth

KW - central metabolism

KW - starvation

KW - central metabolism

KW - expansion growth

KW - organic uptake

KW - plant heterotrophic culture

KW - plant suspension culture

KW - starvation

KW - sucrose

UR - https://www.mendeley.com/catalogue/e526bbfd-c6ae-32a5-b836-94ce91a82d67/

U2 - 10.3390/plants13020277

DO - 10.3390/plants13020277

M3 - Review article

C2 - 38256830

VL - 13

JO - Plants

JF - Plants

SN - 2223-7747

IS - 2

M1 - 277

ER -